Neoplastic-like transformation effect of single-walled and multi-walled-carbon nanotubes compared to asbestos on human lung small airway epithelial cells

Details

• Personal Author:
  Castranova, Vincent ; Derk R ; Meighan T ; Mishra A ; Rojanasakul Y ; Stueckle, Todd A. ; Wang L
• Description:
  Accumulating evidence indicates that carbon nanotubes (CNTs) are biopersistent and can cause lung damage. With similar fibrous morphology and mode of exposure to asbestos, a known human carcinogen, growing concern has arisen for elevated risk of CNT-induced lung carcinogenesis; however, relatively little is known about the long-term carcinogenic effect of CNT. Neoplastic transformation is a key early event leading to carcinogenesis. We studied the ability of single- and multi-walled CNTs to induce neoplastic transformation of human lung epithelial cells compared to asbestos. Long-term (6 month) exposure of the cells to occupationally relevant concentrations of CNT in culture caused a neoplastic-like transformation phenotype as demonstrated by increased cell proliferation, anchorage-independent growth, invasion and angiogenesis. Whole genome expression signature and protein expression analyses showed that single- and multi-walled CNTs shared similar signaling signatures which were distinct from asbestos. These results provide novel toxicogenomic information and suggest distinct particle-associated mechanisms of neoplasia promotion induced by CNTs and asbestos. [Description provided by NIOSH]
• Subjects:
  Carcinogens Fibrosis Lung Lung Diseases Men Occupational Health Particulate Matter Respiratory System Respiratory Tract Diseases Risk Assessment Risk Factors Safety Women

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• Keywords:
  Author Keywords: Lung Cell-biology Cell-function Cellular-function Exposure-levels Fibrous-bodies Genome Expression Humans Invasion Lung-disease Lung-function Microarray Nanotechnology Neoplastic Transformation Risk-factors

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• ISSN:
  1743-5390
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  485-507
• Volume:
  8
• Issue:
  5
• NIOSHTIC Number:
  nn:20042545
• Citation:
  Nanotoxicology 2013 Aug; 8(5):485-507
• Contact Point Address:
  Liying Wang, HELD/PPRB, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505
• Email:
  lmw6@cdc.gov
• CAS Registry Number:
  Asbestos (CAS RN 1332-21-4)
• Federal Fiscal Year:
  2013
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  True
• Source Full Name:
  Nanotoxicology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:f435f80a20f753165780a82b23ce9b3ea55189dd2eaa4c51f0aa038df9fdf948d8aba779d1d4374165810bf07e156202a838dbfd85526a2d9ad4cbfad6206582
• Download URL:
  https://stacks.cdc.gov/view/cdc/194734/cdc_194734_DS1.pdf
• File Type:
  [PDF - 907.46 KB ]