CDC STACKS serves as an archival repository of CDC-published products including scientific findings, journal articles, guidelines, recommendations, or other public health information authored or co-authored by CDC or funded partners.
As a repository, CDC STACKS retains documents in their original published format to ensure public access to scientific information.
i
A genome-wide CRISPR screen in primary immune cells to dissect regulatory networks
-
Jul 16 2015
-
-
Source: Cell. 162(3):675-686.
[PDF-2.33 MB]
Details:
-
Alternative Title:Cell
-
Personal Author:
-
Description:Finding the components of cellular circuits and determining their functions systematically remains a major challenge in mammalian cells. Here, we introduced genome-wide pooled CRISPR-Cas9 libraries into dendritic cells (DCs) to identify genes that control the induction of tumor necrosis factor (Tnf) by bacterial lipopolysaccharide (LPS), a key process in the host response to pathogens, mediated by the Tlr4 pathway. We found many of the known regulators of Tlr4 signaling, as well as dozens of previously unknown candidates that we validated. By measuring protein markers and mRNA profiles in DCs that are deficient in known or candidate genes, we classified the genes into three functional modules with distinct effects on the canonical responses to LPS and highlighted functions for the PAF complex and oligosaccharyltransferase (OST) complex. Our findings uncover new facets of innate immune circuits in primary cells and provide a genetic approach for dissection of mammalian cell circuits.
-
Subjects:
-
Source:
-
Pubmed ID:26189680
-
Pubmed Central ID:PMC4522370
-
Document Type:
-
Funding:
-
Volume:162
-
Issue:3
-
Collection(s):
-
Main Document Checksum:
-
Download URL:
-
File Type:
Supporting Files
-
xlsx
xlsx
xlsx
bin
gif
jpeg
gif
jpeg
gif
jpeg
pdf
gif
jpeg
gif
jpeg
xlsx
pdf
pdf
pdf
pdf
pdf
xlsx
