Utility of Oral Swab Sampling for Ebola Virus Detection in Guinea Pig Model

Spengler, Jessica R.; Chakrabarti, Ayan K.; Coleman-McCray, JoAnn D.; Martin, Brock E.; Nichol, Stuart T.; Spiropoulou, Christina F.; Bird, Brian H.

Back to Previous Page

- Add this to...
- Twitter
- Facebook
- Google Bookmarks
- LinkedIn

Utility of Oral Swab Sampling for Ebola Virus Detection in Guinea Pig Model

Published Date:

Oct 2015

Source:

Emerg Infect Dis. 21(10):1816-1819

Language:

English

[PDF-433.47 KB]

Details:

Personal Authors:

Spengler, Jessica R. ; Chakrabarti, Ayan K. ; Coleman-McCray, JoAnn D. ; Martin, Brock E. ; Nichol, Stuart T. ; ... More ▼

Keywords:

[+]

Pubmed ID:

26401603

Pubmed Central ID:

PMC4593453

Description:

To determine the utility of oral swabs for diagnosing infection with Ebola virus, we used a guinea pig model and obtained daily antemortem and postmortem swab samples. According to quantitative reverse transcription PCR analysis, the diagnostic value was poor for antemortem swab samples but excellent for postmortem samples.

Document Type:

Journal Article

Collection(s):

Emerging Infectious Diseases

Main Document Checksum:

[+]

Supporting Files:

	15-0840-F.gif	image/gif
	15-0840-F.jpg	image/jpeg
	15-0840.nxml	text/xml

No Related Documents.

You May Also Like:

Ebola virus disease diagnostics, Sierra Leone: analysis of real-time RT-PCR values in clinical blood and oral swab specimens

Personal Author:

Erickson, Bobbie R.; Sealy, Tara K.; Flietstra, Tim; Morgan, Laura; Kargbo, Brima; Matt-Lebby, Victor E.; Gibbons, Aridth; Chakrabarti, Ayan K.; Graziano, James; Presser, Lance; Flint, Mike; Bird, Brian H.; Brown, Shelley; Klena, John D.; Blau, Dianna M.; Brault, Aaron C.; Belser, Jessica A.; Salzer, Johanna S.; Schuh, Amy J.; Lo, Michael; Zivcec, Marko; Priestley, Rachael A.; Pyle, Meredith; Goodman, Christin; Bearden, Scott; Amman, Brian R.; Basile, Alison; Bergeron, Éric; Bowen, Michael D.; Dodd, Kimberly A.; Freeman, Molly M.; McMullan, Laura K.; Paddock, Christopher D.; Russell, Brandy J.; Sanchez, Angela J.; Towner, Jonathan S.; Wang, David; Zemtsova, Galina E.; Stoddard, Robyn A.; Turnsek, Maryann; Guerrero, Lisa Wiggleton; Emery, Shannon L.; Stovall, Janae; Kainulainen, Markus H.; Perniciaro, Jamie L.; Mijatovic-Rustempasic, Slavica; Shakirova, Gulchekhra; Winter, Jörn; Sexton, Christopher; Liu, Feng; Slater, Kimetha; Anderson, Raydel; Andersen, Lauren; Chiang, Cheng-Feng; Tzeng, Wen-Pin; Crowe, Samuel J.; Maenner, Matthew J.; Spiropoulou, Christina F.; Nichol, Stuart T.; Ströher, Ute;

Published:

Aug 31 2016

Source:

J Infect Dis. 214(3):S258-S262.

Description:

During the Ebola virus outbreak of 2013-2016, the Viral Special Pathogens Branch field laboratory in Sierra Leone tested approximately 26 000 specimens between August 2014 and October 2015. Analysis of the B2M endogenous control Ct values showed its ...

File Type:

[PDF - 400.26 KB]
Perspectives on West Africa Ebola Virus Disease Outbreak, 2013–2016

Personal Author:

Spengler, Jessica R.; Ervin, Elizabeth D.; Towner, Jonathan S.; Rollin, Pierre E.; Nichol, Stuart T.;

Published:

Jun 15 2016

Source:

Emerg Infect Dis. 22(6):956-963.

Description:

The variety of factors that contributed to the initial undetected spread of Ebola virus disease in West Africa during 2013-2016 and the difficulty controlling the outbreak once the etiology was identified highlight priorities for disease prevention, ...

File Type:

[PDF - 2.38 MB]
Monitoring of Ebola Virus Makona Evolution through Establishment of Advanced Genomic Capability in Liberia

Personal Author:

Kugelman, Jeffrey R.; Wiley, Michael R.; Mate, Suzanne; Ladner, Jason T.; Beitzel, Brett; Fakoli, Lawrence; Taweh, Fahn; Prieto, Karla; Diclaro, Joseph W.; Minogue, Timothy; Schoepp, Randal J.; Schaecher, Kurt E.; Pettitt, James; Bateman, Stacey; Fair, Joseph; Kuhn, Jens H.; Hensley, Lisa; Park, Daniel J.; Sabeti, Pardis C.; Sanchez-Lockhart, Mariano; Bolay, Fatorma K.; Palacios, Gustavo;

Corporate Author:

on behalf of US Army Medical Research Institute of Infectious Diseases. National Institutes of Health. Integrated Research Facility–Frederick Ebola Response Team 2014–2015.

Published:

Jul 2015

Source:

Emerg Infect Dis. 21(7):1135-1143

Description:

To support Liberia's response to the ongoing Ebola virus (EBOV) disease epidemic in Western Africa, we established in-country advanced genomic capabilities to monitor EBOV evolution. Twenty-five EBOV genomes were sequenced at the Liberian Institute f...

File Type:

[PDF - 2.47 MB]
Ebola Virus RNA Stability in Human Blood and Urine in West Africa’s Environmental Conditions

Personal Author:

Janvier, Frédéric; Delaune, Deborah; Poyot, Thomas; Valade, Eric; Mérens, Audrey; Rollin, Pierre E.; Foissaud, Vincent;

Published:

Feb 2016

Source:

Emerg Infect Dis. 22(2):292-294.

Description:

We evaluated RNA stability of Ebola virus in EDTA blood and urine samples collected from infected patients and stored in West Africa's environmental conditions. In blood, RNA was stable for at least 18 days when initial cycle threshold values were <3...

File Type:

[PDF - 439.37 KB]
Hendra Virus Vaccine, a One Health Approach to Protecting Horse, Human, and Environmental Health

Personal Author:

Middleton, Deborah; Pallister, Jackie; Klein, Reuben; Feng, Yan-Ru; Haining, Jessica; Arkinstall, Rachel; Frazer, Leah; Huang, Jin-An; Edwards, Nigel; Wareing, Mark; Elhay, Martin; Hashmi, Zia; Bingham, John; Yamada, Manabu; Johnson, Dayna; White, John; Foord, Adam; Heine, Hans G.; Marsh, Glenn A.; Broder, Christopher C.; Wang, Lin-Fa;

Published:

Mar 2014

Source:

Emerg Infect Dis. 20(3):372-379.

Description:

In recent years, the emergence of several highly pathogenic zoonotic diseases in humans has led to a renewed emphasis on the interconnectedness of human, animal, and environmental health, otherwise known as One Health. For example, Hendra virus (HeV)...

File Type:

[PDF - 416.23 KB]