Details:

Alternative Title:
PLoS Negl Trop Dis
Publisher's site:
http://dx.doi.org/10.1371/journal.pntd.0004085
Personal Author:
Debarba, João Antonio ; Monteiro, Karina Mariante ; Moura, Hercules ; Barr, John R. ; Ferreira, Henrique Bunselmeyer ; ... More +
Debarba, João Antonio ; Monteiro, Karina Mariante ; Moura, Hercules ; Barr, John R. ; Ferreira, Henrique Bunselmeyer ; Zaha, Arnaldo Less -
Description:
Background

The proteins responsible for the key molecular events leading to the structural changes between the developmental stages of Echinococcus granulosus remain unknown. In this work, azidohomoalanine (AHA)-specific labeling was used to identify proteins expressed by E. granulosus protoscoleces (PSCs) upon the induction of strobilar development.

Methodology/Principal Findings

The in vitro incorporation of AHA with different tags into newly synthesized proteins (NSPs) by PSCs was analyzed using SDS-PAGE and confocal microscopy. The LC-MS/MS analysis of AHA-labeled NSPs by PSCs undergoing strobilation allowed for the identification of 365 proteins, of which 75 were differentially expressed in comparison between the presence or absence of strobilation stimuli and 51 were expressed exclusively in either condition. These proteins were mainly involved in metabolic, regulatory and signaling processes.

Conclusions/Significance

After the controlled-labeling of proteins during the induction of strobilar development, we identified modifications in protein expression. The changes in the metabolism and the activation of control and signaling pathways may be important for the correct parasite development and be target for further studies.


Subjects:
[+]
Research Article
Source:
PLoS Negl Trop Dis. 9(9).
Pubmed ID:
26393918
Pubmed Central ID:
PMC4578768
Document Type:
Journal Article
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:4ed04e800d128ed6ea8fef80f766f7d0709663ad63f79d5aac77077009cd23c1
Download URL:
https://stacks.cdc.gov/view/cdc/34694/cdc_34694_DS1.pdf
File Type:

Supporting Files

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  	license.txt	txt
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