Comparison of target labeling methods for use with Affymetrix GeneChips

Details

• Alternative Title:
  BMC Biotechnol
• Personal Author:
  Lonergan, William ; Whistler, Toni ; Vernon, Suzanne D
• Description:
  Background
  
  Several different commercial one-cycle labeling kits are available for preparation of the target for use with the Affymetrix GeneChip platform. However, there have been no evaluations of these different kits to determine if comparable results were generated. We report on the cRNA target synthesis, labeling efficiency and hybridization results using the One-Cycle Target Labeling Assay™ (Affymetrix), the BioArray RNA Amplification and Labeling System™ (Enzo Life Sciences), and the Superscript RNA Amplification System (Invitrogen Life Technologies).
  
  Results
  
  The only notable difference between kits was in the yield of cRNA target synthesized during in vitro transcription, where the BioArray assay had to be repeated several times in order to have sufficient target. However, each kit resulted in comparable signal and detection calls when hybridized to the Affymetrix GeneChip.
  
  Conclusion
  
  These 3 one-cycle labeling kits produce comparable hybridization results. This provides users with several kit options and flexibility when using the Affymetrix system.
  
  
• Subjects:
  Evaluation Studies As Topic Gene Expression Profiling Methodology Article Oligonucleotide Array Sequence Analysis Reagent Kits, Diagnostic RNA, Complementary Sensitivity And Specificity Staining And Labeling
• Source:
  BMC Biotechnol. 2007; 7:24.
• Document Type:
  Journal Article
• Volume:
  7
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:43948cccee2a9d2e515527998625efeb6166e804bd9d0ce9d2dc548e4d44e09f
• Download URL:
  https://stacks.cdc.gov/view/cdc/3463/cdc_3463_DS1.pdf
• File Type:
  [PDF - 595.98 KB ]