Exercise responsive genes measured in peripheral blood of women with Chronic Fatigue Syndrome and matched control subjects

Details

• Alternative Title:
  BMC Physiol
• Personal Author:
  Whistler, Toni ; Jones, James F ; Unger, Elizabeth R ; Vernon, Suzanne D
• Description:
  Background
  
  Chronic fatigue syndrome (CFS) is defined by debilitating fatigue that is exacerbated by physical or mental exertion. To search for markers of CFS-associated post-exertional fatigue, we measured peripheral blood gene expression profiles of women with CFS and matched controls before and after exercise challenge.
  
  Results
  
  Women with CFS and healthy, age-matched, sedentary controls were exercised on a stationary bicycle at 70% of their predicted maximum workload. Blood was obtained before and after the challenge, total RNA was extracted from mononuclear cells, and signal intensity of the labeled cDNA hybridized to a 3800-gene oligonucleotide microarray was measured. We identified differences in gene expression among and between subject groups before and after exercise challenge and evaluated differences in terms of Gene Ontology categories.
  
  Conclusion
  
  These results highlight the potential use of an exercise challenge combined with microarray gene expression analysis in identifying gene ontologies associated with CFS.
  
  
• Subjects:
  Bicycling Case-Control Studies Exercise Fatigue Syndrome, Chronic Female Gene Expression Profiling Humans Oligonucleotide Array Sequence Analysis
• Source:
  BMC Physiol. 5:5.
• Document Type:
  Journal Article
• Volume:
  5
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:d743fad512e9c4b4c49a05fab27eb34cda51755f0e553994d474f4e10c3656f7
• Download URL:
  https://stacks.cdc.gov/view/cdc/3465/cdc_3465_DS1.pdf
• File Type:
  [PDF - 464.49 KB ]