Exercise responsive genes measured in peripheral blood of women with Chronic Fatigue Syndrome and matched control subjects

Details

• Alternative Title:
  BMC Physiol
• Personal Author:
  Whistler, Toni ; Jones, James F ; Unger, Elizabeth R ; Vernon, Suzanne D
• Description:
  Background ; Chronic fatigue syndrome (CFS) is defined by debilitating fatigue that is exacerbated by physical or mental exertion. To search for markers of CFS-associated post-exertional fatigue, we measured peripheral blood gene expression profiles of women with CFS and matched controls before and after exercise challenge. ; Results ; Women with CFS and healthy, age-matched, sedentary controls were exercised on a stationary bicycle at 70% of their predicted maximum workload. Blood was obtained before and after the challenge, total RNA was extracted from mononuclear cells, and signal intensity of the labeled cDNA hybridized to a 3800-gene oligonucleotide microarray was measured. We identified differences in gene expression among and between subject groups before and after exercise challenge and evaluated differences in terms of Gene Ontology categories. ; Conclusion ; These results highlight the potential use of an exercise challenge combined with microarray gene expression analysis in identifying gene ontologies associated with CFS.
• Subjects:
  • Bicycling
  • Case-Control Studies
  • Exercise
  • Fatigue Syndrome, Chronic
  • Female
  • Gene Expression Profiling
  • Humans
  • Oligonucleotide Array Sequence Analysis
• Source:
  BMC Physiol. 5:5.
• Document Type:
  Journal Article
• Volume:
  5
• Download URL:
  https://stacks.cdc.gov/view/cdc/3465/cdc_3465_DS1.pdf
• File Type:
  [PDF - 464.49 KB]
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha-512:bea63a031dfc1384fcd875fd120ea1030d4cd604ed61e466e2a3d4a175d6835c3c701b3edab29547824cffb371b4a006bce63a17c9e8625e1345d888a2826919