Total cholesterol performance of Abell–Levy–Brodie–Kendall reference measurement procedure: Certification of Japanese in-vitro diagnostic assay manufacturers through CDC’s Cholesterol Reference Method Laboratory Network☆

Details

• Alternative Title:
  Clin Chim Acta
• Personal Author:
  Nakamura, Masakazu ; Iso, Hiroyasu ; Kitamura, Akihiko ; Imano, Hironori ; Kiyama, Masahiko ; Yokoyama, Shinji ; Kayamori, Yuzo ; Koyama, Isao ; Nishimura, Kunihiro ; Nakai, Michikazu ; Dasti, Mahnaz ; Vesper, Hubert W. ; Teramoto, Tamio ; Miyamoto, Yoshihiro
• Description:
  Background
  
  Accurate measurement of total cholesterol (TC) is important for cardiovascular disease risk management. The US Centers for Disease Control and Prevention (CDC) and Cholesterol Reference Method Laboratory Network (CRMLN) perform Abell–Levy–Brodie–Kendall (AK) reference measurement procedure (RMP) for TC as a secondary reference method, and implement Certification Protocol for Manufacturers. Japanese CRMLN laboratory at Osaka performed the AK RMP for 22 years, and conducted TC certification for reagent/calibrator/instrument systems of six Japanese manufacturers every 2 years for 16 years. Osaka TC performance was examined and compared to CDC’s reference values.
  
  Methods
  
  AK RMP involved sample hydrolysis, cholesterol extraction, and determination of cholesterol levels by spectrophotometry. The Certification Protocol for Manufacturers includes comparison with AK RMP using at least 40 fresh specimens. Demonstration of average bias ≤3% and total coefficient of variation ≤3% qualified an analytical system for certification.
  
  Results
  
  In the AK RMP used in the Osaka CRMLN laboratory, the regression equation for measuring TC was y (Osaka) = 1.000x (CDC) + 0.032 (n = 619, R2 = 1.000). Six Japanese manufacturers had allowable performance for certification.
  
  Conclusions
  
  The AK RMP for TC measurement was accurate, precise, and stable for 22 years. Six Japanese manufacturers were certified for 16 years.
  
  
• Subjects:
  Biological Assay Certification Cholesterol Humans Japan Laboratories Quality Control Reagent Kits, Diagnostic Reference Standards Reference Values Reproducibility Of Results Sensitivity And Specificity

  More +
• Keywords:
  AK RMP CDC CRMLN TC
• Source:
  Clin Chim Acta. 445:127-132
• Pubmed ID:
  25818239
• Pubmed Central ID:
  PMC4579524
• Document Type:
  Journal Article
• Funding:
  CC999999/Intramural CDC HHSUnited States/
• Volume:
  445
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:ca4b56ccfb6249d39bdb424336bc525d79a00efe0b5ee878b2e32ec955d1bc22
• Download URL:
  https://stacks.cdc.gov/view/cdc/34572/cdc_34572_DS1.pdf
• File Type:
  [PDF - 817.05 KB ]