An electrochemiluminescence assay for analysis of rabies virus glycoprotein content in rabies vaccines

Smith, Todd G.; Ellison, James A.; Ma, Xiaoyue; Kuzmina, Natalia; Carson, William C.; Rupprecht, Charles E.

doi:10.1016/j.vaccine.2013.05.081

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An electrochemiluminescence assay for analysis of rabies virus glycoprotein content in rabies vaccines

Published Date:

Jun 03 2013

Publisher's site:

http://dx.doi.org/10.1016/j.vaccine.2013.05.081 New Site Icon

Source:

Vaccine. 31(33):3333-3338.

[PDF-257.91 KB]

Details:

Alternative Title:

Vaccine

Personal Author:

Smith, Todd G. ; Ellison, James A. ; Ma, Xiaoyue ; Kuzmina, Natalia ; Carson, William C. ; ... More ▼

Description:

Vaccine potency testing is necessary to evaluate the immunogenicity of inactivated rabies virus (RABV) vaccine preparations before human or veterinary application. Currently, the NIH test is recommended by the WHO expert committee to evaluate RABV vaccine potency. However, numerous disadvantages are inherent concerning cost, number of animals and biosafety requirements. As such, several in vitro methods have been proposed for the evaluation of vaccines based on RABV glycoprotein (G) quality and quantity, which is expected to correlate with vaccine potency. In this study an antigen-capture electrochemiluminescent (ECL) assay was developed utilizing anti-RABV G monoclonal antibodies (MAb) to quantify RABV G. One MAb 2-21-14 was specific for a conformational epitope so that only immunogenic, natively folded G was captured in the assay. MAb 2-21-14 or a second MAb (62-80-6) that binds a linear epitope was used for detection of RABV G. Vaccine efficacy was also assessed in vivo using pre-exposure vaccination of mice. Purified native RABV G induced a RABV neutralizing antibody (rVNA) response with a geometric mean titer of 4.2IU/ml and protected 100% of immunized mice against RABV challenge, while an experimental vaccine with a lower quality and quantity of G induced a rVNA titer<0.05IU/ml and protected <50% of immunized mice. These preliminary results support the hypothesis that in vivo immunogenicity may be predicted from the in vitro measurement of RABV G using an ECL assay. Based upon these results, the ECL assay may have utility in replacement of the NIH test.

Subject:

[+]

Pubmed ID:

23742991

Pubmed Central ID:

PMC4568986

Document Type:

Journal Article

Funding:

CC999999/Intramural CDC HHS/United States

Collection(s):

CDC Public Access

Main Document Checksum:

[+]

Supporting Files:

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