An electrochemiluminescence assay for analysis of rabies virus glycoprotein content in rabies vaccines

Smith, Todd G.; Ellison, James A.; Ma, Xiaoyue; Kuzmina, Natalia; Carson, William C.; Rupprecht, Charles E.

doi:10.1016/j.vaccine.2013.05.081

Back to Previous Page

- Add this to...
- Twitter
- Facebook
- Google Bookmarks
- LinkedIn

An electrochemiluminescence assay for analysis of rabies virus glycoprotein content in rabies vaccines

Published Date:

Jun 03 2013

Publisher's site:

http://dx.doi.org/10.1016/j.vaccine.2013.05.081 New Site Icon

Source:

Vaccine. 31(33):3333-3338.

[PDF-257.91 KB]

Details:

Alternative Title:

Vaccine

Personal Author:

Smith, Todd G. ; Ellison, James A. ; Ma, Xiaoyue ; Kuzmina, Natalia ; Carson, William C. ; ... More ▼

Description:

Vaccine potency testing is necessary to evaluate the immunogenicity of inactivated rabies virus (RABV) vaccine preparations before human or veterinary application. Currently, the NIH test is recommended by the WHO expert committee to evaluate RABV vaccine potency. However, numerous disadvantages are inherent concerning cost, number of animals and biosafety requirements. As such, several in vitro methods have been proposed for the evaluation of vaccines based on RABV glycoprotein (G) quality and quantity, which is expected to correlate with vaccine potency. In this study an antigen-capture electrochemiluminescent (ECL) assay was developed utilizing anti-RABV G monoclonal antibodies (MAb) to quantify RABV G. One MAb 2-21-14 was specific for a conformational epitope so that only immunogenic, natively folded G was captured in the assay. MAb 2-21-14 or a second MAb (62-80-6) that binds a linear epitope was used for detection of RABV G. Vaccine efficacy was also assessed in vivo using pre-exposure vaccination of mice. Purified native RABV G induced a RABV neutralizing antibody (rVNA) response with a geometric mean titer of 4.2IU/ml and protected 100% of immunized mice against RABV challenge, while an experimental vaccine with a lower quality and quantity of G induced a rVNA titer<0.05IU/ml and protected <50% of immunized mice. These preliminary results support the hypothesis that in vivo immunogenicity may be predicted from the in vitro measurement of RABV G using an ECL assay. Based upon these results, the ECL assay may have utility in replacement of the NIH test.

Subject:

[+]

Pubmed ID:

23742991

Pubmed Central ID:

PMC4568986

Document Type:

Journal Article

Funding:

CC999999/Intramural CDC HHS/United States

Collection(s):

CDC Public Access

Main Document Checksum:

[+]

Supporting Files:

	nihms-720622-f0001.gif	image/gif
	nihms-720622-f0001.jpg	image/jpeg
	nihms-720622-f0002.gif	image/gif
	nihms-720622-f0002.jpg	image/jpeg
	nihms-720622-f0003.gif	image/gif
	nihms-720622-f0003.jpg	image/jpeg
	nihms-720622.nxml	application/octet-stream

No Related Documents.

You May Also Like:

Assessment of the immunogenicity of rabies vaccine preserved by vaporization and delivered to the duodenal mucosa of gray foxes (Urocyon cinereoargenteus)

Personal Author:

Smith, Todd G.; Wu, Xianfu; Ellison, James A.; Wadhwa, Ashutosh; Franka, Richard; Langham, Gregory L.; Skinner, Brianna L.; Hanlon, Cathleen A.; Bronshtein, Victor L.;

Published Date:

Jun 2017

Source:

Am J Vet Res. 78(6):752-756.

Description:

OBJECTIVETo assess the immunogenicity of thermostable live-attenuated rabies virus (RABV) preserved by vaporization (PBV) and delivered to the duodenal mucosa of a wildlife species targeted by an oral vaccination program.ANIMALS8 gray foxes (Urocyon ...

File Type:

[PDF - 236.92 KB]
Evaluation of Immune Responses in Dogs to Oral Rabies Vaccine under Field Conditions*

Personal Author:

Smith, Todd G.; Millien, Max; Vos, Ad; Fracciterne, Franso Acky; Crowdis, Kelly; Chirodea, Cornelius; Medley, Alexandra; Chipman, Richard; Qin, Yunlong; Blanton, Jesse; Wallace, Ryan;

Published Date:

October 17 2017

Source:

Vaccine. 37(33):4743-4749

Description:

During the 20th century parenteral vaccination of dogs at central-point locations was the foundation of successful canine rabies elimination programs in numerous countries. However, countries that remain enzootic for canine rabies have lower infrastr...

Public Access
Available on:

August 02, 2020
MMWR. Morbidity and Mortality Weekly Report, Vol. 68, no. 32, August 16, 2019

Corporate Authors:

Centers for Disease Control and Prevention (U.S.)

Published Date:

August 16, 2019

Series:

MMWR. Morbidity and mortality weekly report ; v. 68, no. 32

Description:

Contact Lens Health Week — August 19–23, 2019 – Healthy Contact Lens Behaviors Communicated by Eye Care Providers and Recalled by Patients — United States, 2018 / Nuadum M. Konne, MPH; Sarah A. Collier, MPH; Jennifer Spangler, MBA, MPH; et al...

File Type:

[PDF - 509.42 KB]
Infectivity of attenuated poxvirus vaccine vectors and immunogenicity of a raccoonpox vectored rabies vaccine in the Brazilian Free-tailed bat (Tadarida brasiliensis)

Personal Author:

Stading, Ben R.; Osorio, Jorge E.; Velasco-Villa, Andres; Smotherman, Michael; Kingstad-Bakke, Brock; Rocke, Tonie E.;

Published Date:

Sep 17 2016

Source:

Vaccine. 34(44):5352-5358.

Description:

Bats (Order Chiroptera) are an abundant group of mammals with tremendous ecological value as insectivores and plant dispersers, but their role as reservoirs of zoonotic diseases has received more attention in the last decade. With the goal of managin...

File Type:

[PDF - 503.91 KB]
Compendium of animal rabies prevention and control, 2000. National Association of State Public Health Veterinarians, Inc.

Corporate Authors:

National Association of State Public Health Veterinarians, Inc.

Published Date:

July 14, 2000

Source:

MMWR. Recommendations and reports : Morbidity and mortality weekly report. Recommendations and reports / Centers for Disease Control. 2000; 49(RR-8):21-30.

Description:

The purpose of this Compendium is to provide rabies information to veterinarians, public health officials, and others concerned with rabies prevention and control. These recommendations serve as the basis for animal rabies-control programs throughout...

Status:

superseded

File Type:

[PDF - 209.78 KB]