Laser-assisted skin delivery of immunocontraceptive rabies nanoparticulate vaccine in poloxamer gel

Details

• Alternative Title:
  Eur J Pharm Sci
• Personal Author:
  Bansal, Amit ; Gamal, Wael ; Menon, Ipshita Jayaprakash ; Olson, Victoria ; Wu, Xianfu ; D’Souza, Martin J.
• Description:
  A painless skin delivery of vaccine for disease prevention is of great advantage in improving compliance in patients. To test this idea as a proof of concept, we utilized a pDNA vaccine construct, pDNAg333-2GnRH that has a dual function of controlling rabies and inducing immunocontraception in animals. The pDNA was administered to mice in a nanoparticulate form delivered through the skin using the P.L.E.A.S.E.® (Precise Laser Epidermal System) microporation laser device. Laser application was well tolerated, and mild skin reaction was healed completely in 8 days. We demonstrated that adjuvanted nanoparticulate pDNA vaccine significantly upregulated the expression of co-stimulatory molecules in dendritic cells. After topical administration of the adjuvanted nano-vaccine in mice, the high avidity serum for GnRH antibodies were induced and maintained up to 9 weeks. The induced immune response was of a mixed Th1/Th2 profile as measured by IgG subclasses (IgG2a and IgG1) and cytokine levels (IFN-γ and IL-4). Using flow cytometry, we revealed an increase of CD8| T-cells and CD45R B cells upon the administration of the adjuvanted vaccine. Our previous study used the same pDNA nanoparticulate vaccine through an IM route, and a comparable immune response was induced using P.L.E.A.S.E. However, the vaccine dose in the current study was four-fold less than what was applied through the IM route.We concluded that laser-assisted skin vaccination has a potential of becoming a safe and reliable vaccination tool for rabies vaccination in animals or even in humans for pre- or post-exposure prophylaxis.
• Subjects:
  Adjuvants, Immunologic Animals CD8-Positive T-Lymphocytes Humans Lasers Mice Mice, Inbred BALB C Poloxamer Rabies Rabies Vaccines Vaccination

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• Keywords:
  Adjuvant Laser Microporation Particulate Vaccine Rabies Vaccines Skin Vaccination
• Source:
  Eur J Pharm Sci. 155:105560
• Pubmed ID:
  32949750
• Pubmed Central ID:
  PMC10964170
• Document Type:
  Journal Article
• Funding:
  CC999999/ImCDC/Intramural CDC HHSUnited States/
• Volume:
  155
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:dd6f7305b0aabe5633d17f829ed6ab77c42599bfdcd9bef8b0e3ef7978189219
• Download URL:
  https://stacks.cdc.gov/view/cdc/153556/cdc_153556_DS1.pdf
• File Type:
  [PDF - 1.43 MB ]