Details:

Alternative Title:
PLoS One
Publisher's site:
http://dx.doi.org/10.1371/journal.pone.0137108
Personal Author:
Fatykhova, Diana ; Rabes, Anne ; Machnik, Christoph ; Guruprasad, Kunchur ; Pache, Florence ; ... More +
Fatykhova, Diana ; Rabes, Anne ; Machnik, Christoph ; Guruprasad, Kunchur ; Pache, Florence ; Berg, Johanna ; Toennies, Mario ; Bauer, Torsten T. ; Schneider, Paul ; Schimek, Maria ; Eggeling, Stephan ; Mitchell, Timothy J. ; Mitchell, Andrea M. ; Hilker, Rolf ; Hain, Torsten ; Suttorp, Norbert ; Hippenstiel, Stefan ; Hocke, Andreas C. ; Opitz, Bastian ; Less -
Description:
Streptococcus pneumoniae is a major cause of pneumonia, sepsis and meningitis. The pore-forming toxin pneumolysin is a key virulence factor of S. pneumoniae, which can be sensed by the NLRP3 inflammasome. Among the over 90 serotypes, serotype 1 pneumococci (particularly MLST306) have emerged across the globe as a major cause of invasive disease. The cause for its particularity is, however, incompletely understood. We therefore examined pneumococcal infection in human cells and a human lung organ culture system mimicking infection of the lower respiratory tract. We demonstrate that different pneumococcal serotypes differentially activate inflammasome-dependent IL-1β production in human lung tissue and cells. Whereas serotype 2, 3, 6B, 9N pneumococci expressing fully haemolytic pneumolysins activate NLRP3 inflammasome-dependent responses, serotype 1 and 8 strains expressing non-haemolytic toxins are poor activators of IL-1β production. Accordingly, purified haemolytic pneumolysin but not serotype 1-associated non-haemolytic toxin activates strong IL-1β production in human lungs. Our data suggest that the evasion of inflammasome-dependent innate immune responses by serotype 1 pneumococci might contribute to their ability to cause invasive diseases in humans.
Subject:
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Research Article

Source:
PLoS One. 2015 ; 10(8).;
Pubmed ID:
26317436
Pubmed Central ID:
PMC4552725
Document Type:
Journal Article ;
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:e4cddb39d1bf8aaea55346e6857857d320176ea8e107b6cf93a925194f4e9545
File Type:

Supporting Files

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