THE STABILITY OF MARKERS IN DRIED-BLOOD SPOTS FOR RECOMMENDED NEWBORN SCREENING DISORDERS IN THE UNITED STATES
Published Date:Sep 21 2011
Source:Clin Biochem. 44(0):1445-1450.
Dried Blood Spot Testing
Pubmed Central ID:PMC4557772
Funding:CC999999/Intramural CDC HHS/United States
We aimed to measure separately the contributions of heat and humidity to changes in levels of 34 markers of inborn disorders in dried-blood-spot (DBS) samples.
Design and Methods
Paired sets of DBSs were stored at 37°C for predetermined intervals in low-humidity and high-humidity environments. Marker levels of all samples in each complete sample set were measured in a single analytic run.
During the 30±5 day study, galactose-1-phosphate uridyltransferase and biotinidase lost almost 65% of initial activities in low-humidity storage; most of the degradation in 27 other markers was attributable to adverse effects of high humidity storage; seven markers in DBSs stored at high humidity lost more than 90% of initial levels by the end of the study and four of the seven lost more than 50% of initial levels within the first week of storage.
Minimizing both humidity and temperature in the DBS transportation and storage environments is essential to maintaining sample integrity.
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