THE STABILITY OF MARKERS IN DRIED-BLOOD SPOTS FOR RECOMMENDED NEWBORN SCREENING DISORDERS IN THE UNITED STATES

Details

• Alternative Title:
  Clin Biochem
• Personal Author:
  Adam, BW ; Hall, EM ; Sternberg, M ; Lim, TH ; Flores, SR ; O’Brien, S ; Simms, D ; Li, LX ; De Jesus, VR ; Hannon, WH
• Description:
  Objective
  
  We aimed to measure separately the contributions of heat and humidity to changes in levels of 34 markers of inborn disorders in dried-blood-spot (DBS) samples.
  
  Design and Methods
  
  Paired sets of DBSs were stored at 37°C for predetermined intervals in low-humidity and high-humidity environments. Marker levels of all samples in each complete sample set were measured in a single analytic run.
  
  Results
  
  During the 30±5 day study, galactose-1-phosphate uridyltransferase and biotinidase lost almost 65% of initial activities in low-humidity storage; most of the degradation in 27 other markers was attributable to adverse effects of high humidity storage; seven markers in DBSs stored at high humidity lost more than 90% of initial levels by the end of the study and four of the seven lost more than 50% of initial levels within the first week of storage.
  
  Conclusions
  
  Minimizing both humidity and temperature in the DBS transportation and storage environments is essential to maintaining sample integrity.
  
  
• Subjects:
  Arginine Biomarkers Biotinidase Carnitine Dried Blood Spot Testing Enzyme Stability Heptanoates Humans Humidity Infant, Newborn Myristic Acids Neonatal Screening Preservation, Biological Protein Stability United States UTP-Hexose-1-Phosphate Uridylyltransferase

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• Keywords:
  Disorder Markers Dried-blood Spots Humidity Effects Newborn Screening Sample Stability Storage Conditions Temperature Effects
• Source:
  Clin Biochem. 44(0):1445-1450
• Pubmed ID:
  21963384
• Pubmed Central ID:
  PMC4557772
• Document Type:
  Journal Article
• Funding:
  CC999999/Intramural CDC HHSUnited States/
• Volume:
  44
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha-512:5a4265a13db5f5206f032d520a5c6995eca37849d4b7db616195c126be621bc7090a4b9551cc639fe515e8743776594217ad5a3e0a37af97bd01e0bab2749930
• Download URL:
  https://stacks.cdc.gov/view/cdc/34048/cdc_34048_DS1.pdf
• File Type:
  [PDF - 1.06 MB ]