Stabilities of hemoglobins A and S in dried blood spots stored under controlled conditions
Published Date:May 20 2013
Source:Clin Biochem. 46(12):1089-1092.
Chromatography, High Pressure Liquid
Dried Blood Spots
Dried Blood Spot Testing
Sickle Cell Disease
Pubmed Central ID:PMC4557610
Funding:CC999999/Intramural CDC HHS/United States
We aimed to measure separately the contributions of heat and humidity to changes in levels of hemoglobins A and S in dried-blood-spot (DBS) samples.
Design and methods
We stored paired sets of DBSs at 37 °C for predetermined intervals in low-humidity and high-humidity environments. Hemoglobin A and S levels of all samples in each complete set were measured in a single high performance liquid chromatography run.
During the one-month storage intervals, both hemoglobin species lost about 35% of initial levels in low-humidity storage and almost all of initial levels in high-humidity storage.
Minimizing both humidity and temperature in the transportation and storage environments of DBS samples is essential to maintaining the integrity of the hemoglobin tetramer molecules.
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