Stabilities of hemoglobins A and S in dried blood spots stored under controlled conditions

Details

• Alternative Title:
  Clin Biochem
• Personal Author:
  Adam, Barbara W. ; Chafin, Dana L. ; De Jesús, Victor R.
• Description:
  Objective
  
  We aimed to measure separately the contributions of heat and humidity to changes in levels of hemoglobins A and S in dried-blood-spot (DBS) samples.
  
  Design and methods
  
  We stored paired sets of DBSs at 37 °C for predetermined intervals in low-humidity and high-humidity environments. Hemoglobin A and S levels of all samples in each complete set were measured in a single high performance liquid chromatography run.
  
  Results
  
  During the one-month storage intervals, both hemoglobin species lost about 35% of initial levels in low-humidity storage and almost all of initial levels in high-humidity storage.
  
  Conclusions
  
  Minimizing both humidity and temperature in the transportation and storage environments of DBS samples is essential to maintaining the integrity of the hemoglobin tetramer molecules.
  
  
• Subjects:
  Adult Chromatography, High Pressure Liquid Dried Blood Spot Testing Environment, Controlled Hemoglobin, Sickle Hemoglobin A Humans Humidity Infant, Newborn Preservation, Biological Protein Stability Proteolysis

  More +
• Keywords:
  Dried Blood Spots Hemoglobin S Hemoglobin Stability Newborn Screening Sickle Cell Disease Storage Humidity Storage Temperature
• Source:
  Clin Biochem. 46(12):1089-1092
• Pubmed ID:
  23701845
• Pubmed Central ID:
  PMC4557610
• Document Type:
  Journal Article
• Funding:
  CC999999/ImCDC/Intramural CDC HHSUnited States/
• Volume:
  46
• Issue:
  12
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha-512:edc74b0f4392f1e4ac3c89d61f376c4f169a4db861732c95f64fce6fdbd4ec83ebc867a1149fa6764309474bcdb5a0d2efe5fc3c1c22bd23a2467ab96b138f89
• Download URL:
  https://stacks.cdc.gov/view/cdc/34024/cdc_34024_DS1.pdf
• File Type:
  [PDF - 292.76 KB ]