Expression pattern of oxidative stress and antioxidant defense-related genes in the aging Fischer 344/NHsd rat cochlea

Details

• Alternative Title:
  Neurobiol Aging
• Personal Author:
  Tanaka, Chiemi ; Coling, Donald E. ; Manohar, Senthilvelan ; Chen, Guang-Di ; Hu, Bo Hua ; Salvi, Richard ; Henderson, Donald
• Description:
  The biological mechanisms that give rise to age-related hearing loss (ARHL) are still poorly understood. However, there is growing recognition that oxidative stress may be an important factor. To address this issue, we measured the changes in the expression of cochlear oxidative stress and antioxidant defense-related genes in young (2 months old), middle-aged (12 months old), and old (21-25 months old) Fischer 344/NHsd (F344/NHsd) rats and compared gene expression changes with ARHL. A quantitative real-time reverse transcription polymerase chain reaction array revealed a significant age-related downregulation of only 1 gene, stearoyl-coenzyme A desaturase 1, and upregulation of 12 genes: 24-dehydrocholesterol reductase; aminoadipate-semialdehyde synthase; cytoglobin; dual oxidase 2; glutathione peroxidase 3; glutathione peroxidase 6; glutathione S-transferase, kappa 1; glutathione reductase; nicotinamide adenine dinucleotide phosphate (NAD(P)H) dehydrogenase, quinone 1; solute carrier Family 38, Member 5; thioredoxin interacting protein; and vimentin. Statistical analyses revealed significant correlations between gene expression and auditory function in 8 genes. Our results identified specific subsets of oxidative stress genes that appear to play an important role in ARHL in the Fischer 344/NHsd rat.
• Subjects:
  Aging Animals Antioxidants Gene Expression Regulation, Developmental Male Oxidative Stress Rats Rats, Inbred F344 Reactive Oxygen Species
• Source:
  Neurobiol Aging. 33(8):1842.e1-1842.14.
• Pubmed ID:
  22300951
• Pubmed Central ID:
  PMC4077169
• Document Type:
  Journal Article
• Funding:
  1R01OH008113/OH/NIOSH CDC HHS/United States ; R01 DC010154/DC/NIDCD NIH HHS/United States
• Volume:
  33
• Issue:
  8
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:af96f35726f27f55e9503d8b3ec814931c3a608379d2cd5308b1a21e60027d91
• Download URL:
  https://stacks.cdc.gov/view/cdc/33695/cdc_33695_DS1.pdf
• File Type:
  [PDF - 477.67 KB ]