Vitamin D Treatment Modulates Organic Dust-Induced Cellular and Airway Inflammatory Consequences
Published Date:Dec 20 2012
Source:J Biochem Mol Toxicol. 27(1):77-86.
Disease Models, Animal
In Vitro Techniques
Mice, Inbred C57BL
Protein Kinase C
Toll-Like Receptor 2
Toll-Like Receptor 4
Pubmed Central ID:PMC4004104
Funding:1U54OH010162-01/OH/NIOSH CDC HHS/United States
R01 ES019325/ES/NIEHS NIH HHS/United States
R01: ES019325/ES/NIEHS NIH HHS/United States
Description:Exposure to organic dusts elicits airway inflammatory diseases. Vitamin D recently has been associated with various airway inflammatory diseases, but its role in agricultural organic dust exposures is unknown. This study investigated whether vitamin D reduces organic dust-induced inflammatory outcomes in cell culture and animal models. Organic dust extracts obtained from swine confinement facilities induced neutrophil chemokine production (human IL-8, murine CXCL1/CXCL2). Neutrophil chemokine induction was reduced in human blood monocytes, human bronchial epithelial cells, and murine lung slices pretreated with 1,25-(OH)(2) D(3) . Intranasal inhalation of organic dust extract induced neutrophil influx, and CXCL1/CXCL2 release was also decreased in mice fed a relatively high vitamin D diet as compared to mice fed a low vitamin D diet. These findings were associated with reduced tracheal epithelial cell PKCα and PKCε activity and whole lung TLR2 and TLR4 gene expression. Collectively, vitamin D plays a role in modulating organic dust-induced airway inflammatory outcomes.
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