Vitamin D Treatment Modulates Organic Dust-Induced Cellular and Airway Inflammatory Consequences

Details

• Alternative Title:
  J Biochem Mol Toxicol
• Personal Author:
  Golden, Gregory A. ; Wyatt, Todd A. ; Romberger, Debra J. ; Reiff, Daniel ; McCaskill, Michael ; Bauer, Christopher ; Gleason, Angela M. ; Poole, Jill A.
• Description:
  Exposure to organic dusts elicits airway inflammatory diseases. Vitamin D recently has been associated with various airway inflammatory diseases, but its role in agricultural organic dust exposures is unknown. This study investigated whether vitamin D reduces organic dust-induced inflammatory outcomes in cell culture and animal models. Organic dust extracts obtained from swine confinement facilities induced neutrophil chemokine production (human IL-8, murine CXCL1/CXCL2). Neutrophil chemokine induction was reduced in human blood monocytes, human bronchial epithelial cells, and murine lung slices pretreated with 1,25-(OH)(2) D(3) . Intranasal inhalation of organic dust extract induced neutrophil influx, and CXCL1/CXCL2 release was also decreased in mice fed a relatively high vitamin D diet as compared to mice fed a low vitamin D diet. These findings were associated with reduced tracheal epithelial cell PKCα and PKCε activity and whole lung TLR2 and TLR4 gene expression. Collectively, vitamin D plays a role in modulating organic dust-induced airway inflammatory outcomes.
• Subjects:
  Administration, Intranasal Animals Cell Line Chemokine CXCL1 Chemokine CXCL2 Chemokines Disease Models, Animal Dust Humans Inflammation Interleukin-8 In Vitro Techniques Lung Male Mice Mice, Inbred C57BL Monocytes Organic Agriculture Protein Kinase C Swine Toll-Like Receptor 2 Toll-Like Receptor 4 Vitamin D

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• Source:
  J Biochem Mol Toxicol. 27(1):77-86.
• Pubmed ID:
  23281135
• Pubmed Central ID:
  PMC4004104
• Document Type:
  Journal Article
• Funding:
  1U54OH010162-01/OH/NIOSH CDC HHS/United States ; R01 ES019325/ES/NIEHS NIH HHS/United States ; R01: ES019325/ES/NIEHS NIH HHS/United States
• Volume:
  27
• Issue:
  1
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:8c53c752bfd6519d24a7fbdc4d943accab41302bbe4a4a7b78e7d5c202737b2a
• Download URL:
  https://stacks.cdc.gov/view/cdc/33682/cdc_33682_DS1.pdf
• File Type:
  [PDF - 1.53 MB ]