Competitive immunochromatographic assay for the detection of the organophosphorus pesticide chlorpyrifos

Details

• Alternative Title:
  Anal Chim Acta
• Personal Author:
  Kim, Young Ah ; Lee, Eun-Hye ; Kim, Kwang-Ok ; Lee, Yong Tae ; Hammock, Bruce D. ; Lee, Hye-Sung
• Description:
  An immunochromatographic assay (ICA) based on competitive antigen-coated format using colloidal gold as the label was developed for the detection of the organophosphorus insecticide chlorpyrifos. The ICA test strip consisted of a membrane with a detection zone, a sample pad and an absorbent pad. The membrane was separately coated with chlorpyrifos Hapten-OVA conjugate (test line) and anti-mouse IgG (control line). Based on the fact that the competition is between the migrating analyte in the sample and the analyte hapten immobilized on the test strip for the binding sites of the antibody-colloidal gold (Ab-CG) conjugate migrating on the test strip, this study suggests that the relative migration speed between the two migrating substances is a critically important factor for the sensitive detection by competitive ICA. This criterion was utilized for the confirmation of appropriateness of a nitrocellulose (NC) membrane for chlorpyrifos ICA. The detection limit of the ICA for chlorpyrifos standard and chlorpyrifos spiked into agricultural samples were 10 and 50 ng mL(-1), respectively. The assay time for the ICA test was less than 10 min, suitable for rapid on-site testing of chlorpyrifos.
• Subjects:
  • Animals
  • Antibodies, Monoclonal
  • Binding Sites
  • Binding, Competitive
  • Chlorpyrifos
  • Collodion
  • Cross Reactions
  • Gold
  • Haptens
  • Immunoassay
  More +
• Keywords:
  • Organophosphorus pesticide
  • Chlorpyrifos
  • Immunochromatographic assay
• Source:
  Anal Chim Acta. 2011; 693(0):106-113
• Pubmed ID:
  21504817
• Pubmed Central ID:
  PMC3927651
• Document Type:
  Journal Article
• Funding:
  U50 OH007550/OH/NIOSH CDC HHSUnited States/ ; P42 ES004699/ES/NIEHS NIH HHSUnited States/ ; U54 OH007550/OH/NIOSH CDC HHSUnited States/ ; OH07550/OH/NIOSH CDC HHSUnited States/ ; U54OH007550/ACL/ACL HHSUnited States/
• Volume:
  693
• Issue:
  0
• Download URL:
  https://stacks.cdc.gov/view/cdc/33662/cdc_33662_DS1.pdf
• File Type:
  [PDF - 1.57 MB]
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:174b11edcd54cc25ad28dbfa1e088d92389bc855b5133d7cc7b60ed9b6478fcd