Details:

Alternative Title:
Anal Chim Acta
Publisher's site:
http://dx.doi.org/10.1016/j.aca.2011.03.011
Personal Author:
Kim, Young Ah ; Lee, Eun-Hye ; Kim, Kwang-Ok ; Lee, Yong Tae ; Hammock, Bruce D. ; ... More +
Kim, Young Ah ; Lee, Eun-Hye ; Kim, Kwang-Ok ; Lee, Yong Tae ; Hammock, Bruce D. ; Lee, Hye-Sung ; Less -
Description:
An immunochromatographic assay (ICA) based on competitive antigen-coated format using colloidal gold as the label was developed for the detection of the organophosphorus insecticide chlorpyrifos. The ICA test strip consisted of a membrane with a detection zone, a sample pad and an absorbent pad. The membrane was separately coated with chlorpyrifos Hapten-OVA conjugate (test line) and anti-mouse IgG (control line). Based on the fact that the competition is between the migrating analyte in the sample and the analyte hapten immobilized on the test strip for the binding sites of the antibody-colloidal gold (Ab-CG) conjugate migrating on the test strip, this study suggests that the relative migration speed between the two migrating substances is a critically important factor for the sensitive detection by competitive ICA. This criterion was utilized for the confirmation of appropriateness of a nitrocellulose (NC) membrane for chlorpyrifos ICA. The detection limit of the ICA for chlorpyrifos standard and chlorpyrifos spiked into agricultural samples were 10 and 50 ng mL(-1), respectively. The assay time for the ICA test was less than 10 min, suitable for rapid on-site testing of chlorpyrifos.
Subject:
[+]
Animals
Antibodies, Monoclonal
Article
Binding, Competitive
Binding Sites
Chlorpyrifos
Collodion
Cross Reactions
Gold
Haptens
Immunoassay
Immunochromatographic Assay
Insecticides
Mice
Organophosphorus Pesticide
Ovalbumin

Source:
Anal Chim Acta. 2011; 693(0):106-113.
Pubmed ID:
21504817
Pubmed Central ID:
PMC3927651
Document Type:
Journal Article ;
Funding:
OH07550/OH/NIOSH CDC HHS/United States ; P42 ES004699/ES/NIEHS NIH HHS/United States ; P42 ES004699/ES/NIEHS NIH HHS/United States ;
OH07550/OH/NIOSH CDC HHS/United States ; P42 ES004699/ES/NIEHS NIH HHS/United States ; P42 ES004699/ES/NIEHS NIH HHS/United States ; Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:174b11edcd54cc25ad28dbfa1e088d92389bc855b5133d7cc7b60ed9b6478fcd
File Type:

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