Competitive immunochromatographic assay for the detection of the organophosphorus pesticide chlorpyrifos

Kim, Young Ah; Lee, Eun-Hye; Kim, Kwang-Ok; Lee, Yong Tae; Hammock, Bruce D.; Lee, Hye-Sung

doi:10.1016/j.aca.2011.03.011

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Competitive immunochromatographic assay for the detection of the organophosphorus pesticide chlorpyrifos

Published Date:

Mar 11 2011

Publisher's site:

http://dx.doi.org/10.1016/j.aca.2011.03.011 New Site Icon

Source:

Anal Chim Acta. 2011; 693(0):106-113.

[PDF-1.57 MB]

Details:

Personal Authors:

Kim, Young Ah ; Lee, Eun-Hye ; Kim, Kwang-Ok ; Lee, Yong Tae ; Hammock, Bruce D. ; ... More ▼

Keywords:

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Pubmed ID:

21504817

Pubmed Central ID:

PMC3927651

Description:

An immunochromatographic assay (ICA) based on competitive antigen-coated format using colloidal gold as the label was developed for the detection of the organophosphorus insecticide chlorpyrifos. The ICA test strip consisted of a membrane with a detection zone, a sample pad and an absorbent pad. The membrane was separately coated with chlorpyrifos Hapten-OVA conjugate (test line) and anti-mouse IgG (control line). Based on the fact that the competition is between the migrating analyte in the sample and the analyte hapten immobilized on the test strip for the binding sites of the antibody-colloidal gold (Ab-CG) conjugate migrating on the test strip, this study suggests that the relative migration speed between the two migrating substances is a critically important factor for the sensitive detection by competitive ICA. This criterion was utilized for the confirmation of appropriateness of a nitrocellulose (NC) membrane for chlorpyrifos ICA. The detection limit of the ICA for chlorpyrifos standard and chlorpyrifos spiked into agricultural samples were 10 and 50 ng mL(-1), respectively. The assay time for the ICA test was less than 10 min, suitable for rapid on-site testing of chlorpyrifos.

Document Type:

Journal Article

Collection(s):

CDC Public Access

Funding:

OH07550/OH/NIOSH CDC HHS/United States
P42 ES004699/ES/NIEHS NIH HHS/United States
P42 ES004699/ES/NIEHS NIH HHS/United States

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