Competitive immunochromatographic assay for the detection of the organophosphorus pesticide chlorpyrifos

Details

• Personal Author:
  Hammock BD ; Kim K-O ; Kim YA ; Lee E-H ; Lee H-S ; Lee YT
• Description:
  An immunochromatographic assay (ICA) based on competitive antigen-coated format using colloidal gold as the label was developed for the detection of the organophosphorus insecticide chlorpyrifos. The ICA test strip consisted of a membrane with a detection zone, a sample pad and an absorbent pad. The membrane was separately coated with chlorpyrifos Hapten-OVA conjugate (test line) and anti-mouse IgG (control line). Based on the fact that the competition is between the migrating analyte in the sample and the analyte hapten immobilized on the test strip for the binding sites of the antibody-colloidal gold (Ab-CG) conjugate migrating on the test strip, this study suggests that the relative migration speed between the two migrating substances is a critically important factor for the sensitive detection by competitive ICA. This criterion was utilized for the confirmation of appropriateness of a nitrocellulose (NC) membrane for chlorpyrifos ICA. The detection limit of the ICA for chlorpyrifos standard and chlorpyrifos spiked into agricultural samples were 10 and 50 ng mL(-1), respectively. The assay time for the ICA test was less than 10 min, suitable for rapid on-site testing of chlorpyrifos. [Description provided by NIOSH]
• Subjects:
  • Antibodies
  • Biological Assay
  • Environmental Monitoring
  • Insecticides
  • Occupational Health
  • Organophosphates
  • Safety
• Keywords:
  • Antibody-response
  • Bioassays
  • Exposure-assessment
  • Immunological-tests
  • Insecticides
  • Organo-phosphorus-pesticides
  • Pesticides-and-agricultural-chemicals
  • Author Keywords: Organophosphorus pesticide
  • Chlorpyrifos
  • Immunochromatographic assay
• ISSN:
  0003-2670
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  106-113
• Volume:
  693
• Issue:
  1
• NIOSHTIC Number:
  nn:20039328
• Citation:
  Anal Chim Acta 2011 May; 693(1-2):106-113
• Contact Point Address:
  Hye-Sung Lee, Department of Food Science and Nutrition, Kyungpook National University, Daegu 702-701, Republic of Korea
• Email:
  hslee@knu.ac.kr
• CAS Registry Number:
  • Chlorpyrifos (CAS RN 2921-88-2)
• Federal Fiscal Year:
  2011
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of California - Davis
• Peer Reviewed:
  True
• Start Date:
  20010930
• Source Full Name:
  Analytica Chimica Acta
• End Date:
  20270929
• Download URL:
  https://stacks.cdc.gov/view/cdc/192672/cdc_192672_DS1.pdf
• File Type:
  [PDF - 553.78 KB]
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:b910d2ae32f5a026fe1b726b649cea28f08848572b83abc53e430879a1407d623a10e2f2dc4001aa0eb788fd780533e12b422870d65e8b6e820b8935e744425e