Label-Free Polypeptide-Based Enzyme Detection Using a Graphene-Nanoparticle Hybrid Sensor

Details

• Alternative Title:
  Adv Mater
• Personal Author:
  Myung, Sung ; Yin, Perry T. ; Kim, Cheoljin ; Park, Jaesung ; Solanki, Aniruddh ; Reyes, Pavel Ivanoff ; Lu, Yicheng ; Kim, Kwang S. ; Lee, Ki-Bum
• Description:
  A graphene-nanoparticle (NP) hybrid biosensor that utilizes an electrical hysteresis change to detect the enzymatic activity and concentration of Carboxypeptidase B was developed. The results indicate that the novel graphene-NP hybrid biosensor, utilizing electrical hysteresis, has the ability to detect concentrations of targeted enzyme on the micromolar scale. Furthermore, to the knowledge of the authors, this is the first demonstration of a graphene-based biosensor that utilizes a hysteresis change resulting from metallic NPs assembled on a graphene surface.
• Subjects:
  Article Biosensing Techniques Carboxypeptidase B Conductometry Enzyme Activation Equipment Design Equipment Failure Analysis Graphite Nanoparticles Nanotechnology Peptides Staining And Labeling

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• Source:
  Adv Mater. 2012; 24(45):6081-6087
• Pubmed ID:
  22961629
• Pubmed Central ID:
  PMC3626444
• Document Type:
  Journal Article
• Funding:
  DP2 OD006462/OD/NIH HHS/United States ; T32 GM008339/GM/NIGMS NIH HHS/United States ; 1DP20D006462-01/DP/NCCDPHP CDC HHS/United States
• Volume:
  24
• Issue:
  45
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:4bc2d40dce75f3ef4207476fee20319471756e14a02bcd60be9bc9d70fc03566
• Download URL:
  https://stacks.cdc.gov/view/cdc/33540/cdc_33540_DS1.pdf
• File Type:
  [PDF - 2.51 MB ]