22961629

PMC3626444

1DP20D006462-01/DP/NCCDPHP CDC HHS/United States
DP2 OD006462/OD/NIH HHS/United States
T32 GM008339/GM/NIGMS NIH HHS/United States

CDC Public Access

A graphene-nanoparticle (NP) hybrid biosensor that utilizes an electrical hysteresis change to detect the enzymatic activity and concentration of Carboxypeptidase B was developed. The results indicate that the novel graphene-NP hybrid biosensor, utilizing electrical hysteresis, has the ability to detect concentrations of targeted enzyme on the micromolar scale. Furthermore, to the knowledge of the authors, this is the first demonstration of a graphene-based biosensor that utilizes a hysteresis change resulting from metallic NPs assembled on a graphene surface.