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Electrophoretic build-up of multi nanoparticle array for a highly sensitive immunoassay
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Sep 06 2012
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Source: Biosens Bioelectron. 2012; 41:302-308.
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Alternative Title:Biosens Bioelectron
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Description:One of the challenges in shrinking immunoassays to smaller sizes is to immobilize the biological molecules to nanometer-scaled spots. To overcome this complication, we have employed a particle-based immunoassay to create a nanostructured platform with a regular array of sensing elements. The technique makes use of an electrophoretic particle entrapment system (EPES) to immobilize nanoparticles that are coated with biological reagents into wells using a very small trapping potential. To provide useful information for controlling the trapping force and optimal design of the nanoarray, electrophoretic trapping of a nanoparticle was modeled numerically. The trapping efficiency, defined as the fraction of wells occupied by a single particle, was 91%. The performance of the array was demonstrated with a competitive immunoassay for a small molecule analyte, 3-phenoxybenzoic acid (214.2 g mole(-1)). The limit of detection determined with a basic fluorescence microscope was 0.006 μg l(-1) (30 pM); this represented a sixteen-fold improvement in sensitivity compared to a standard 96-well plate-based ELISA; the improvement was attributed to the small size of the sample volume and the presence of light diffraction among factors unique to this structure. The EPES/nanoarray system promises to offer a new standard in applications that require portable, point-of-care and real-time monitoring with high sensitivity.
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Pubmed ID:23021853
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Pubmed Central ID:PMC3525773
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Volume:41
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[PDF-1.08 MB]
