Molecular events associated with epithelial to mesenchymal transition of nasopharyngeal carcinoma cells in the absence of Epstein-Barr virus genome

Details

• Alternative Title:
  J Biomed Sci
• Personal Author:
  Lin, Jung-Chung ; Liao, Shuen-Kuei ; Lee, En-Huei ; Hung, Man-Shan ; Sayion, Yiyang ; Chen, Hung-Chang ; Kang, Chen-Chen ; Huang, Liang-Sheng ; Cherng, Jaw-Ming
• Description:
  Epithelial-mesenchymal transition (EMT) is an important process in tumor metastasis. The EMT-related events associated with metastasis of NPC in the absence of EBV have not been elucidated. We established an EBV-negative NPC cell line from a bone marrow biopsy of an NPC patient. Using a Matrigel system we isolated an invasive and non-invasive sublines, designated NPC-BM29 and NPC-BM00. NPC-BM29 acquired an invasive-like phenotype characterized by EMT, marked by down-regulation of E-cadherin and beta-catenin with concomitant increased expression of Ets1. NPC-BM29 cells expressed >or= 10-fold higher of MMP-9 than NPC-BM00 cells. NPC-BM29 cells grew better in 2% serum than NPC-BM00 cells, with a population doubling-time of 26.8 h and 30.7 h, respectively. A marked reduction in colony-formation ability of NPC-BM00 cells compared to NPC-BM29 was observed. Wound-healing assay revealed that NPC-BM29 cells displayed higher motility than NPC-BM00 and the motility was further enhanced by cell treatment with TPA, a PKC activator. Cell surface markers and tumor-associated molecules, AE3, MAK6 and sialyl-Tn, were up-regulated in NPC-BM29 cells, whereas the expression of HLA-DR and CD54 was significantly increased in NPC-BM00 cells. NPC-BM29 consistently released higher levels of IL-8 and IL-10 than NPC-BM00, with low levels of IL-1alpha expression in both cell lines. Higher level of VEGF production was detected in NPC-BM00 than NPC-BM29 cells. These data show that EBV is not required for exhibiting multiple metastatic phenotypes associated with EMT. More studies that target right molecules/signalings associated with the EMT may offer new therapeutic intervention options for NPC invasion and metastasis.
• Subjects:
  Biopsy Bone Marrow Cells Carcinoma Cell Adhesion Cell Line, Tumor Cell Proliferation Epithelium Genome, Viral Herpesvirus 4, Human Humans Mesoderm Nasopharyngeal Neoplasms Neoplasm Metastasis Phenotype Research Wound Healing

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• Source:
  J Biomed Sci. 2009; 16(1):105.
• Document Type:
  Journal Article
• Volume:
  16
• Issue:
  1
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:46323b7d3331222c4687acf28ad9b54a0f70d79110c73a7931b20cec7a48adb1
• Download URL:
  https://stacks.cdc.gov/view/cdc/3354/cdc_3354_DS1.pdf
• File Type:
  [PDF - 2.08 MB ]