Simultaneous determination of multiple phthalate metabolites and bisphenol-A in human urine by liquid chromatography-tandem mass spectrometry

Details

• Alternative Title:
  J Chromatogr B Analyt Technol Biomed Life Sci
• Personal Author:
  Chen, Mei ; Tao, Lin ; Collins, Erin M. ; Austin, Christine ; Lu, Chensheng
• Description:
  Phthalates and bisphenol A are environmental endocrine-disrupting chemicals used widely in common consumer products. There is increasing concern about human exposure to phthalates and bisphenol A due to the potential adverse effects related to the anti-androgenic activity of phthalates and estrogenic activity of bisphenol A. In assessing environmental exposure to phthalates and bisphenol A, it is essential to have a validated analytical method that can quantify trace concentrations of phthalate metabolites and bisphenol A in humans. In this study, we developed and validated an accurate, sensitive, and robust LC-MS/MS method to simultaneously quantify 5 phthalate monoester metabolites, including mono-methyl phthalate, mono-ethyl phthalate, mono-butyl phthalate, mono-benzyl phthalate, mono-2-ethylhexyl phthalate, and bisphenol A in human urine. In this method, the phthalate metabolites and bisphenol A, along with their isotope labeled internal standards, were extracted from 200 μl of human urine using automated off-line solid phase extraction. The analytes were quantitatively determined using LC-MS/MS operated in negative electrospray ionization multiple reaction-monitoring mode. The limit of quantification was 0.3 ng/ml for mono-methyl phthalate, mono-ethyl phthalate, mono-benzyl phthalate and bisphenol A, and 1 ng/ml for mono-butyl phthalate and mono-2-ethylhexyl phthalate. The precision and accuracy were well within the acceptable 15% range. This validated method has been used successfully in assessing exposure to phthalates and bisphenol A in humans.
• Subjects:
  Adult Benzhydryl Compounds Chromatography, Liquid Humans Phenols Phthalic Acids Reproducibility Of Results Sensitivity And Specificity Solid Phase Extraction Tandem Mass Spectrometry
• Source:
  J Chromatogr B Analyt Technol Biomed Life Sci. 2012; 904:73-80.
• Pubmed ID:
  22884473
• Pubmed Central ID:
  PMC3578285
• Document Type:
  Journal Article
• Funding:
  5R21ES019773-02/ES/NIEHS NIH HHS/United States ; 5R21OH009831-02/OH/NIOSH CDC HHS/United States ; ES000002/ES/NIEHS NIH HHS/United States ; R21 ES019773/ES/NIEHS NIH HHS/United States
• Volume:
  904
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:ca73edd77c1834ad20658f1145268d8852fe907c3e97e1eb6d3c5f3f3c1877d2
• Download URL:
  https://stacks.cdc.gov/view/cdc/33521/cdc_33521_DS1.pdf
• File Type:
  [PDF - 564.29 KB ]