Biomarkers of Exposure to Triclocarban in Urine and Serum

Details

• Alternative Title:
  Toxicology
• Personal Author:
  Ye, Xiaoyun ; Zhou, Xiaoliu ; Furr, Johnathan ; Ahn, Ki Chang ; Hammock, Bruce D. ; Gray, Earl L. ; Calafat, Antonia M.
• Description:
  3,4,4'-Trichlorocarbanilide (triclocarban, TCC) is widely used as an antimicrobial agent in a variety of consumer and personal care products. TCC is considered a potential endocrine disruptor, but its potential toxic effects in humans are still largely unknown. Because of its widespread uses, the potential for human exposure to TCC is high. In order to identify adequate exposure biomarkers of TCC, we investigated the metabolic profile of TCC in adult female Sprague Dawley rats after administering TCC once (500 mg/kg body weight) by oral gavage. Urine was collected 0-24 h before dosing, and 0-24 h and 24-48 h after dosing. Serum was collected at necropsy 48 h after dosing. We identified several metabolites of TCC in urine and serum by on-line solid phase extraction-high performance liquid chromatography-mass spectrometry. We unambiguously identified two major oxidative metabolites of TCC, 3'-hydroxy-TCC and 2'-hydroxy-TCC, by comparing their chromatographic behavior and mass spectral fragmentation patterns with those of authentic standards. By contrast, compared to these oxidative metabolites, we detected very low levels of TCC in the urine or serum. Taken together these data suggest that in rats, oxidation of TCC is a major metabolic pathway. We also measured TCC and its oxidative metabolites in 50 urine and 16 serum samples collected from adults in the United States. The results suggest differences in the metabolic profile of TCC in rats and in humans; oxidation appears to be a minor metabolic pathway in humans. Total (free plus conjugated) TCC could serve as a potential biomarker for human exposure to TCC.
• Subjects:
  Animals Anti-Infective Agents, Local Biomarkers Carbanilides Chromatography, High Pressure Liquid Environmental Exposure Female Humans Mass Spectrometry Oxidation-Reduction Rats Rats, Sprague-Dawley Solid Phase Extraction Species Specificity Time Factors Water Pollutants, Chemical

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• Keywords:
  2’-OH-Triclocarban 3’-OH-Triclocarban Biomarkers Exposure Assessment Oxidative Metabolism Triclocarban
• Source:
  Toxicology. 286(1-3):69-74
• Pubmed ID:
  21635932
• Pubmed Central ID:
  PMC3534749
• Document Type:
  Journal Article
• Funding:
  Y1-ES-80124-01/ES/NIEHS NIH HHSUnited States/ ; U50 OH007550/OH/NIOSH CDC HHSUnited States/ ; PHS OH07550/OH/NIOSH CDC HHSUnited States/ ; R01 ES002710/ES/NIEHS NIH HHSUnited States/ ; P42 ES004699/ES/NIEHS NIH HHSUnited States/ ; R01 ES002710-31/ES/NIEHS NIH HHSUnited States/ ; U54 OH007550/OH/NIOSH CDC HHSUnited States/ ; U54OH007550/ACL/ACL HHSUnited States/
• Volume:
  286
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:e0992bf8d5cd1cc9190cc7c42c7556ea18e5fdc87fbf614444d1d0c5a3a0d2e4
• Download URL:
  https://stacks.cdc.gov/view/cdc/33497/cdc_33497_DS1.pdf
• File Type:
  [PDF - 1.06 MB ]