Asymmetry and aging of mycobacterial cells leads to variable growth and antibiotic susceptibility

Details

• Alternative Title:
  Science
• Personal Author:
  Aldridge, Bree B. ; Fernandez-Suarez, Marta ; Heller, Danielle ; Ambravaneswaran, Vijay ; Irimia, Daniel ; Toner, Mehmet ; Fortune, Sarah
• Description:
  Cells use both deterministic and stochastic mechanisms to generate cell-to-cell heterogeneity, which enables the population to better withstand environmental stress. Here we show that, within a clonal population of mycobacteria, there is deterministic heterogeneity in elongation rate that arises because mycobacteria grow in an unusual, unipolar fashion. Division of the asymmetrically growing mother cell gives rise to daughter cells that differ in elongation rate and size. Because the mycobacterial cell division cycle is governed by time, not cell size, rapidly elongating cells do not divide more frequently than slowly elongating cells. The physiologically distinct subpopulations of cells that arise through asymmetric growth and division are differentially susceptible to clinically important classes of antibiotics.
• Subjects:
  Anti-Bacterial Agents Cell Cycle Cell Division Cell Wall Cycloserine Escherichia Coli Isoniazid Microbial Sensitivity Tests Microfluidic Analytical Techniques Mycobacterium Smegmatis Mycobacterium Tuberculosis Rifampin Thienamycins Time Factors

  More +
• Source:
  Science. 335(6064):100-104.
• Pubmed ID:
  22174129
• Pubmed Central ID:
  PMC3397429
• Document Type:
  Journal Article
• Funding:
  1DP20D001378/DP/NCCDPHP CDC HHS/United States ; DP2 OD001378/OD/NIH HHS/United States ; P30 AI060354/AI/NIAID NIH HHS/United States ; P41 EB002503/EB/NIBIB NIH HHS/United States
• Volume:
  335
• Issue:
  6064
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:c945465b7d5f190d145edeb67de61101c9028d9d15fcdde34a83a3c3d7e6ccea
• Download URL:
  https://stacks.cdc.gov/view/cdc/33401/cdc_33401_DS1.pdf
• File Type:
  [PDF - 1.98 MB ]