Asymmetry and aging of mycobacterial cells leads to variable growth and antibiotic susceptibility

Aldridge, Bree B.; Fernandez-Suarez, Marta; Heller, Danielle; Ambravaneswaran, Vijay; Irimia, Daniel; Toner, Mehmet; Fortune, Sarah

doi:10.1126/science.1216166

Back to Previous Page

- Add this to...
- Twitter
- Facebook
- Google Bookmarks
- LinkedIn

Asymmetry and aging of mycobacterial cells leads to variable growth and antibiotic susceptibility

Published Date:

Dec 15 2011

Publisher's site:

http://dx.doi.org/10.1126/science.1216166 New Site Icon

Source:

Science. 335(6064):100-104.

[PDF-1.98 MB]

Details:

Personal Authors:

Aldridge, Bree B. ; Fernandez-Suarez, Marta ; Heller, Danielle ; Ambravaneswaran, Vijay ; Irimia, Daniel ; ... More ▼

Keywords:

[+]

Pubmed ID:

22174129

Pubmed Central ID:

PMC3397429

Description:

Cells use both deterministic and stochastic mechanisms to generate cell-to-cell heterogeneity, which enables the population to better withstand environmental stress. Here we show that, within a clonal population of mycobacteria, there is deterministic heterogeneity in elongation rate that arises because mycobacteria grow in an unusual, unipolar fashion. Division of the asymmetrically growing mother cell gives rise to daughter cells that differ in elongation rate and size. Because the mycobacterial cell division cycle is governed by time, not cell size, rapidly elongating cells do not divide more frequently than slowly elongating cells. The physiologically distinct subpopulations of cells that arise through asymmetric growth and division are differentially susceptible to clinically important classes of antibiotics.

Document Type:

Journal Article

Collection(s):

CDC Public Access

Funding:

1DP20D001378/DP/NCCDPHP CDC HHS/United States
DP2 OD001378/OD/NIH HHS/United States
P30 AI060354/AI/NIAID NIH HHS/United States
P41 EB002503/EB/NIBIB NIH HHS/United States
P41 EB002503/EB/NIBIB NIH HHS/United States

Supporting Files:

	nihms389397f4.gif	image/gif
	nihms389397f4.jpg	image/jpeg
	NIHMS389397-supplement-Supplementary_Data.pdf	application/pdf
	nihms389397.nxml	text/plain
	nihms389397f1.gif	image/gif
	nihms389397f1.jpg	image/jpeg
	nihms389397f2.gif	image/gif
	nihms389397f2.jpg	image/jpeg
	nihms389397f3.gif	image/gif
	nihms389397f3.jpg	image/jpeg

No Related Documents.

You May Also Like:

Exploitation of Mycobacterium tuberculosis Reporter Strains to Probe the Impact of Vaccination at Sites of Infection

Personal Author:

Sukumar, Neelima; Tan, Shumin; Aldridge, Bree B.; Russell, David G.;

Published:

Sep 18 2014

Source:

PLoS Pathog. 2014; 10(9).

Description:

Mycobacterium tuberculosis (Mtb) remains a major public health problem, with an effective vaccine continuing to prove elusive. Progress in vaccination strategies has been hampered by a lack of appreciation of the bacterium's response to dynamic chang...

File Type:

[PDF - 5.52 MB]
Alveolar Macrophage Innate Response to Mycobacterium immunogenum, the Etiological Agent of Hypersensitivity Pneumonitis: Role of JNK and p38 MAPK Pathways

Personal Author:

Chandra, Harish; Yadav, Ekta; Yadav, Jagjit S.;

Published:

Dec 11 2013

Source:

PLoS One. 2013; 8(12).

Description:

Mycobacterium immunogenum is an emerging pathogen of the immune-mediated lung disease hypersensitivity pneumonitis (HP) reported in machinists occupationally exposed to contaminated metal working fluid (MWF). However, the mechanism of its interaction...

File Type:

[PDF - 2.39 MB]
Diagnostic standards and classification of tuberculosis in adults and children

Corporate Author:

American Thoracic Society, Committee on Revision of Diagnostic Standards.. United States, Dept of Health and Human Services.. United States, Public Health Service.. Centers for Disease Control and Prevention (U.S.).

Published:

April 2000

Source:

Am J Respir Crit Care Med. 2000 Apr;161(4 Pt 1):1376-95.

Series:

American journal of respiratory and critical care medicine ; v. 161

Description:

A joint statement of the American Thoracic Society and the CDC, this article provides a framework for and an understanding of the diagnostic approaches to tuberculosis (TB) infection/disease and to present a classification scheme that facilitates man...

File Type:

[PDF - 151.00 KB]
Antimycobacterial activity of DNA intercalator inhibitors of Mycobacterium tuberculosis primase DnaG

Personal Author:

Gajadeera, Chathurada; Willby, Melisa J.; Green, Keith D.; Shaul, Pazit; Fridman, Micha; Garneau-Tsodikova, Sylvie; Posey, James E.; Tsodikov, Oleg V.;

Published:

Sep 24 2014

Source:

J Antibiot (Tokyo). 68(3):153-157.

Description:

Owing to the rise in drug resistance in tuberculosis combined with the global spread of its causative pathogen, Mycobacterium tuberculosis (Mtb), innovative anti mycobacterial agents are urgently needed. Recently, we developed a novel primase-pyropho...

File Type:

[PDF - 461.45 KB]
Known Unknowns of Cardiolipin Signaling: The Best Is Yet To Come

Personal Author:

Maguire, John J.; Tyurina, Yulia Y.; Mohammadyani, Dariush; Kapralov, Aleksandr A.; Anthonymuthu, Tamil S.; Qu, Feng; Amoscato, Andrew A.; Sparvero, Louis J.; Tyurin, Vladimir A.; Planas-Iglesias, Joan; He, Rong-Rong; Klein-Seetharaman, Judith; Bayır, Hülya; Kagan, Valerian E.;

Published:

Aug 04 2016

Source:

Biochim Biophys Acta. 1862(1):8-24.

Description:

Since its discovery 75years ago, a wealth of knowledge has accumulated on the role of cardiolipin, the hallmark phospholipid of mitochondria, in bioenergetics and particularly on the structural organization of the inner mitochondrial membrane. A surg...

File Type:

[PDF - 3.33 MB]