Asymmetry and aging of mycobacterial cells leads to variable growth and antibiotic susceptibility

Aldridge, Bree B.; Fernandez-Suarez, Marta; Heller, Danielle; Ambravaneswaran, Vijay; Irimia, Daniel; Toner, Mehmet; Fortune, Sarah

doi:10.1126/science.1216166

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Asymmetry and aging of mycobacterial cells leads to variable growth and antibiotic susceptibility

Published Date:

Dec 15 2011

Publisher's site:

http://dx.doi.org/10.1126/science.1216166 New Site Icon

Source:

Science. 335(6064):100-104.

[PDF-1.98 MB]

Details:

Personal Authors:

Aldridge, Bree B. ; Fernandez-Suarez, Marta ; Heller, Danielle ; Ambravaneswaran, Vijay ; Irimia, Daniel ; ... More ▼

Keywords:

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Pubmed ID:

22174129

Pubmed Central ID:

PMC3397429

Description:

Cells use both deterministic and stochastic mechanisms to generate cell-to-cell heterogeneity, which enables the population to better withstand environmental stress. Here we show that, within a clonal population of mycobacteria, there is deterministic heterogeneity in elongation rate that arises because mycobacteria grow in an unusual, unipolar fashion. Division of the asymmetrically growing mother cell gives rise to daughter cells that differ in elongation rate and size. Because the mycobacterial cell division cycle is governed by time, not cell size, rapidly elongating cells do not divide more frequently than slowly elongating cells. The physiologically distinct subpopulations of cells that arise through asymmetric growth and division are differentially susceptible to clinically important classes of antibiotics.

Document Type:

Journal Article

Collection(s):

CDC Public Access

Funding:

1DP20D001378/DP/NCCDPHP CDC HHS/United States
DP2 OD001378/OD/NIH HHS/United States
P30 AI060354/AI/NIAID NIH HHS/United States
P41 EB002503/EB/NIBIB NIH HHS/United States
P41 EB002503/EB/NIBIB NIH HHS/United States

Main Document Checksum:

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Supporting Files:

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