Details:

Alternative Title:
Science
Publisher's site:
http://dx.doi.org/10.1126/science.1216166
Personal Author:
Aldridge, Bree B. ; Fernandez-Suarez, Marta ; Heller, Danielle ; Ambravaneswaran, Vijay ; Irimia, Daniel ; ... More +
Aldridge, Bree B. ; Fernandez-Suarez, Marta ; Heller, Danielle ; Ambravaneswaran, Vijay ; Irimia, Daniel ; Toner, Mehmet ; Fortune, Sarah ; Less -
Description:
Cells use both deterministic and stochastic mechanisms to generate cell-to-cell heterogeneity, which enables the population to better withstand environmental stress. Here we show that, within a clonal population of mycobacteria, there is deterministic heterogeneity in elongation rate that arises because mycobacteria grow in an unusual, unipolar fashion. Division of the asymmetrically growing mother cell gives rise to daughter cells that differ in elongation rate and size. Because the mycobacterial cell division cycle is governed by time, not cell size, rapidly elongating cells do not divide more frequently than slowly elongating cells. The physiologically distinct subpopulations of cells that arise through asymmetric growth and division are differentially susceptible to clinically important classes of antibiotics.
Subject:
[+]
Anti-Bacterial Agents
Article
Cell Cycle
Cell Division
Cell Wall
Cycloserine
Escherichia Coli
Isoniazid
Microbial Sensitivity Tests
Microfluidic Analytical Techniques
Mycobacterium Smegmatis
Mycobacterium Tuberculosis
Rifampin
Thienamycins
Time Factors

Source:
Science. 335(6064):100-104. ;
Pubmed ID:
22174129
Pubmed Central ID:
PMC3397429
Document Type:
Journal Article ;
Funding:
1DP20D001378/DP/NCCDPHP CDC HHS/United States ; DP2 OD001378/OD/NIH HHS/United States ; P30 AI060354/AI/NIAID NIH HHS/United States ; P41 EB002503/EB/NIBIB NIH HHS/United States ; P41 EB002503/EB/NIBIB NIH HHS/United States ;
1DP20D001378/DP/NCCDPHP CDC HHS/United States ; DP2 OD001378/OD/NIH HHS/United States ; P30 AI060354/AI/NIAID NIH HHS/United States ; P41 EB002503/EB/NIBIB NIH HHS/United States ; P41 EB002503/EB/NIBIB NIH HHS/United States ; Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:c945465b7d5f190d145edeb67de61101c9028d9d15fcdde34a83a3c3d7e6ccea
File Type:

Supporting Files

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