Mass-spectrometric characterization of peroxidized and hydrolyzed lipids in plasma and dendritic cells of tumor-bearing animals

Details

• Alternative Title:
  Biochem Biophys Res Commun
• Personal Author:
  Tyurin, Vladimir A. ; Cao, Wei ; Tyurina, Yulia Y. ; Gabrilovich, Dmitry I. ; Kagan, Valerian E.
• Description:
  Dendritic cells are the most potent antigen presenting cells responsible for the development of immune responses in cancer. However, it is known that the function of dendritic cells in tumor-bearing hosts is severely compromised. Our previous studies demonstrated that the defects in dendritic cell function are due, to a large extent, to the accumulation of high amounts of lipids--predominantly triglycerides--in a substantial proportion of dendritic cells in tumor-bearing mice and patients with cancer. The dendritic cells accumulation of lipids is likely associated with their up-regulation of a scavenger receptor A. This receptor is primarily responsible for uptake of modified lipids. Here, by using different versions of liquid chromatography-mass spectrometry, we identified several molecular species of oxygenated lipids in plasma of tumor-bearing animals that may be responsible for their uptake and accumulation by dendritic cells via scavenger receptor A-dependent pathway--the effect that may be associated with the loss of dendritic cell's immune surveillance function in cancer.
• Subjects:
  Animals Cell Line, Tumor Chromatography, Liquid Dendritic Cells Hydrolysis Immunologic Surveillance Lipid Peroxidation Mass Spectrometry Mice Mice, Inbred C57BL Neoplasms Scavenger Receptors, Class A Triglycerides

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• Source:
  Biochem Biophys Res Commun. 2011; 413(1):149-153.
• Pubmed ID:
  21872574
• Pubmed Central ID:
  PMC3356782
• Document Type:
  Journal Article
• Funding:
  HL094488/HL/NHLBI NIH HHS/United States ; HL70755/HL/NHLBI NIH HHS/United States ; OH008282/OH/NIOSH CDC HHS/United States ; R01 HL094488/HL/NHLBI NIH HHS/United States ; R01 HL094488-02/HL/NHLBI NIH HHS/United States ; U19AIO68021/PHS HHS/United States
• Volume:
  413
• Issue:
  1
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:332624f4558ee6aa99c3aff25ab51958adac8114fd96f49313f067ae7e246e52
• Download URL:
  https://stacks.cdc.gov/view/cdc/33365/cdc_33365_DS1.pdf
• File Type:
  [PDF - 1.32 MB ]