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In vivo time-lapse imaging and serial section electron microscopy reveal developmental synaptic rearrangements
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In vivo time-lapse imaging and serial section electron microscopy reveal developmental synaptic rearrangements
Details:
  • Pubmed ID:
    21262466
  • Pubmed Central ID:
    PMC3052740
  • Funding:
    DP1 OD000458/OD/NIH HHS/United States
    DP1 OD000458-01/OD/NIH HHS/United States
    DP1 OD000458-02/OD/NIH HHS/United States
    DP1 OD000458-03/OD/NIH HHS/United States
    DP1 OD000458-04/OD/NIH HHS/United States
    DP1 OD000458-05/OD/NIH HHS/United States
    DP10D000458/DP/NCCDPHP CDC HHS/United States
    EY-011261-14S1/EY/NEI NIH HHS/United States
    R01 EY-011261/EY/NEI NIH HHS/United States
    R01 EY011261/EY/NEI NIH HHS/United States
    R01 EY011261-08/EY/NEI NIH HHS/United States
    R01 EY011261-09/EY/NEI NIH HHS/United States
    R01 EY011261-10/EY/NEI NIH HHS/United States
    R01 EY011261-11/EY/NEI NIH HHS/United States
    R01 EY011261-12/EY/NEI NIH HHS/United States
    R01 EY011261-13/EY/NEI NIH HHS/United States
    R01 EY011261-14/EY/NEI NIH HHS/United States
    R01 EY011261-14S1/EY/NEI NIH HHS/United States
    R01 EY012138/EY/NEI NIH HHS/United States
    R01-EY12138/EY/NEI NIH HHS/United States
  • Document Type:
  • Collection(s):
  • Description:
    Dendrites, axons, and synapses are dynamic during circuit development; however, changes in microcircuit connections as branches stabilize have not been directly demonstrated. By combining in vivo time-lapse imaging of Xenopus tectal neurons with electron microscope reconstructions of imaged neurons, we report the distribution and ultrastructure of synapses on individual vertebrate neurons and relate these synaptic properties to dynamics in dendritic and axonal arbor structure over hours or days of imaging. Dynamic dendrites have a high density of immature synapses, whereas stable dendrites have sparser, mature synapses. Axons initiate contacts from multisynapse boutons on stable branches. Connections are refined by decreasing convergence from multiple inputs to postsynaptic dendrites and by decreasing divergence from multisynapse boutons to postsynaptic sites. Visual deprivation or NMDAR antagonists decreased synapse maturation and elimination, suggesting that coactive input activity promotes microcircuit development by concurrently regulating synapse elimination and maturation of remaining contacts.