CDC STACKS serves as an archival repository of CDC-published products including scientific findings, journal articles, guidelines, recommendations, or other public health information authored or co-authored by CDC or funded partners.
As a repository, CDC STACKS retains documents in their original published format to ensure public access to scientific information.
i
In vivo time-lapse imaging and serial section electron microscopy reveal developmental synaptic rearrangements
-
1 27 2011
-
-
Source: Neuron. 2010; 69(2):273-286
Details:
-
Alternative Title:Neuron
-
Personal Author:
-
Description:Dendrites, axons, and synapses are dynamic during circuit development; however, changes in microcircuit connections as branches stabilize have not been directly demonstrated. By combining in vivo time-lapse imaging of Xenopus tectal neurons with electron microscope reconstructions of imaged neurons, we report the distribution and ultrastructure of synapses on individual vertebrate neurons and relate these synaptic properties to dynamics in dendritic and axonal arbor structure over hours or days of imaging. Dynamic dendrites have a high density of immature synapses, whereas stable dendrites have sparser, mature synapses. Axons initiate contacts from multisynapse boutons on stable branches. Connections are refined by decreasing convergence from multiple inputs to postsynaptic dendrites and by decreasing divergence from multisynapse boutons to postsynaptic sites. Visual deprivation or NMDAR antagonists decreased synapse maturation and elimination, suggesting that coactive input activity promotes microcircuit development by concurrently regulating synapse elimination and maturation of remaining contacts.
-
Subjects:
-
Keywords:
-
Source:
-
Pubmed ID:21262466
-
Pubmed Central ID:PMC3052740
-
Document Type:
-
Funding:
-
Volume:69
-
Issue:2
-
Collection(s):
-
Main Document Checksum:
-
Download URL:
-
File Type:
Supporting Files
-
jpeg gif jpeg gif jpeg gif jpeg gif jpeg gif doc jpeg gif jpeg avi avi avi xml gif jpeg gif