In vivo time-lapse imaging and serial section electron microscopy reveal developmental synaptic rearrangements

Details

• Alternative Title:
  Neuron
• Personal Author:
  Li, Jianli ; Erisir, Alev ; Cline, Hollis
• Description:
  Dendrites, axons, and synapses are dynamic during circuit development; however, changes in microcircuit connections as branches stabilize have not been directly demonstrated. By combining in vivo time-lapse imaging of Xenopus tectal neurons with electron microscope reconstructions of imaged neurons, we report the distribution and ultrastructure of synapses on individual vertebrate neurons and relate these synaptic properties to dynamics in dendritic and axonal arbor structure over hours or days of imaging. Dynamic dendrites have a high density of immature synapses, whereas stable dendrites have sparser, mature synapses. Axons initiate contacts from multisynapse boutons on stable branches. Connections are refined by decreasing convergence from multiple inputs to postsynaptic dendrites and by decreasing divergence from multisynapse boutons to postsynaptic sites. Visual deprivation or NMDAR antagonists decreased synapse maturation and elimination, suggesting that coactive input activity promotes microcircuit development by concurrently regulating synapse elimination and maturation of remaining contacts.
• Subjects:
  Animals Dendrites Microscopy, Electron Synapses Synaptic Vesicles Time-Lapse Imaging Xenopus Laevis
• Keywords:
  3D Reconstruction In Vivo Imaging Retinotectal System Synapse Elimination Synapse Formation Synapse Maturation Ultrastructure Xenopus
• Source:
  Neuron. 2010; 69(2):273-286
• Pubmed ID:
  21262466
• Pubmed Central ID:
  PMC3052740
• Document Type:
  Journal Article
• Funding:
  DP10D000458/DP/NCCDPHP CDC HHSUnited States/ ; R01 EY011261-11/EY/NEI NIH HHSUnited States/ ; DP1 OD000458-02/OD/NIH HHSUnited States/ ; R01 EY011261-14S1/EY/NEI NIH HHSUnited States/ ; DP1 OD000458-03/OD/NIH HHSUnited States/ ; DP1 OD000458/OD/NIH HHSUnited States/ ; R01 EY011261-10/EY/NEI NIH HHSUnited States/ ; R01 EY-011261/EY/NEI NIH HHSUnited States/ ; R01 EY011261-12/EY/NEI NIH HHSUnited States/ ; R01-EY12138/EY/NEI NIH HHSUnited States/ ; DP1 OD000458-05/OD/NIH HHSUnited States/ ; R01 EY011261-08/EY/NEI NIH HHSUnited States/ ; R01 EY011261-13/EY/NEI NIH HHSUnited States/ ; R01 EY012138/EY/NEI NIH HHSUnited States/ ; R01 EY011261-09/EY/NEI NIH HHSUnited States/ ; DP1 OD000458-01/OD/NIH HHSUnited States/ ; EY-011261-14S1/EY/NEI NIH HHSUnited States/ ; R01 EY011261-14/EY/NEI NIH HHSUnited States/ ; R01 EY011261/EY/NEI NIH HHSUnited States/ ; DP1 OD000458-04/OD/NIH HHSUnited States/
• Volume:
  69
• Issue:
  2
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:f7f3ee36852e4fdf21be99d1f110d9163d9d0490a5f24da32781769cee1affa5
• Download URL:
  https://stacks.cdc.gov/view/cdc/33183/cdc_33183_DS1.pdf
• File Type:
  [PDF - 5.86 MB ]