Engineering artificial cells by combining HeLa-based cell-free expression and ultra-thin double emulsion template

Details

• Alternative Title:
  Methods Cell Biol
• Personal Author:
  Ho, Kwun Yin ; Murray, Victoria L. ; Liu, Allen P.
• Description:
  Generation of artificial cells provides the bridge needed to cover the gap between studying the complexity of biological processes in whole cells and studying these same processes in an in vitro reconstituted system. Artificial cells are defined as the encapsulation of biologically active material in a biological or synthetic membrane. Here, we describe a robust and general method to produce artificial cells for the purpose of mimicking one or more behaviors of a cell. A microfluidic double emulsion system is used to encapsulate a mammalian cell-free expression system that is able to express membrane proteins into the bilayer or soluble proteins inside the vesicles. The development of a robust platform that allows the assembly of artificial cells is valuable in understanding subcellular functions and emergent behaviors in a more cell-like environment as well as for creating novel signaling pathways to achieve specific cellular behaviors.
• Subjects:
  Article Artificial Cells Capillary Microfluidics Cell-free Expression Double Emulsion Lipid Bilayer Vesicle Synthetic Biology
• Source:
  Methods Cell Biol. 128:303-318.
• Pubmed ID:
  25997354
• Pubmed Central ID:
  PMC4526213
• Document Type:
  Journal Article
• Funding:
  DP2 HL117748/HL/NHLBI NIH HHS/United States ; DP2 HL117748-01/DP/NCCDPHP CDC HHS/United States
• Volume:
  128
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:75d2337361c14abac77f08bf60a4e15b02be2130f234dee484eb633186a2bd37
• Download URL:
  https://stacks.cdc.gov/view/cdc/32702/cdc_32702_DS1.pdf
• File Type:
  [PDF - 1.13 MB ]