Dual and recombinant infections: an integral part of the HIV-1 epidemic in Brazil.

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Ramos, A. ; Tanuri, A. ; Schechter, M. ; Rayfield, M. A. ; Hu, D. J. ; Cabral, M. C. ; Bandea, C. I. ; Baggs, J. ; Pieniazek, D.
• Description:
  We systematically evaluated multiple and recombinant infections in an HIV-infected population selected for vaccine trials. Seventy-nine HIV-1 infected persons in a clinical cohort study in Rio de Janeiro, Brazil, were evaluated for 1 year. A combination of molecular screening assays and DNA sequencing showed 3 dual infections (3.8%), 6 recombinant infections (7.6%), and 70 (88.6%) infections involving single viral subtypes. In the three dual infections, we identified HIV-1 subtypes F and B, F and D, and B and D; in contrast, the single and recombinant infections involved only HIV-1 subtypes B and F. The recombinants had five distinct B/F mosaic patterns: Bgag-p17/Bgag-p24/Fpol/Benv, Fgag-p17/Bgag-p24/Fpol/Fenv, Bgag-p17/B-Fgag-p24/Fpol/Fenv, Bgag-p17/B-Fgag-p24/Fpol/Benv, and Fgag-p17/B-Fgag-p24/Fpol/Fenv. No association was found between dual or recombinant infections and demographic or clinical variables. These findings indicate that dual and recombinant infections are emerging as an integral part of the HIV/AIDS epidemic in Brazil and emphasize the heterogenous character of epidemics emerging in countries where multiple viral subtypes coexist.
• Subjects:
  Adult Base Sequence Cloning, Molecular Cohort Studies Disease Outbreaks DNA, Viral Female Gene Products, Env Gene Products, Gag HIV-1 HIV Infections HIV Protease Humans Male Middle Aged Molecular Sequence Data Phylogeny Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Prospective Studies Recombination, Genetic Sensitivity And Specificity Sequence Analysis, DNA

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• Source:
  Emerg Infect Dis. 5(1):65-74.
• Document Type:
  Journal Article
• Place as Subject:
  Brazil
• Volume:
  5
• Issue:
  1
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha-512:1ebbdc0d8dc1618fc0eab9dea637a8ce29bdee386d288ec8f44a5978d1fd2e6907430f0e6feff4f930da4915ab844f0f9d98393e976ce24d2b29e9358e71e3f4
• Download URL:
  https://stacks.cdc.gov/view/cdc/3251/cdc_3251_DS1.pdf
• File Type:
  [PDF - 172.86 KB ]