Details:

Alternative Title:
Nucleic Acids Res
Publisher's site:
http://dx.doi.org/10.1093/nar/gku523
Personal Author:
Koh, Hye Ran ; Xing, Li ; Kleiman, Lawrence ; Myong, Sua
Koh, Hye Ran ; Xing, Li ; Kleiman, Lawrence ; Myong, Sua Less -
Description:
Helicases contribute to diverse biological processes including replication, transcription and translation. Recent reports suggest that unwinding of some helicases display repetitive activity, yet the functional role of the repetitiveness requires further investigation. Using single-molecule fluorescence assays, we elucidated a unique unwinding mechanism of RNA helicase A (RHA) that entails discrete substeps consisting of binding, activation, unwinding, stalling and reactivation stages. This multi-step process is repeated many times by a single RHA molecule without dissociation, resulting in repetitive unwinding/rewinding cycles. Our kinetic and mutational analysis indicates that the two double stand RNA binding domains at the N-terminus of RHA are responsible for such repetitive unwinding behavior in addition to providing an increased binding affinity to RNA. Further, the repetitive unwinding induces an efficient annealing of a complementary RNA by making the unwound strand more accessible. The complex and unusual mechanism displayed by RHA may help in explaining how the repetitive unwinding of helicases contributes to their biological functions.
Subjects:
[+]
DEAD-box RNA Helicases HEK293 Cells Humans Nucleic Acid Enzymes Protein Structure, Tertiary RNA, Double-Stranded RNA-Binding Proteins
Source:
Nucleic Acids Res. 2014; 42(13):8556-8564.
Pubmed ID:
24914047
Pubmed Central ID:
PMC4117756
Document Type:
Journal Article
Funding:
1DP2GM105453/DP/NCCDPHP CDC HHS/United States ; DP2 GM105453/GM/NIGMS NIH HHS/United States ; Canadian Institutes of Health Research/Canada
1DP2GM105453/DP/NCCDPHP CDC HHS/United States ; DP2 GM105453/GM/NIGMS NIH HHS/United States ; Canadian Institutes of Health Research/Canada Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:f2d4b76a21e03c08fc5104bbf089ebdcbbdde65e6625cce979bf8755f8fafc4e
Download URL:
https://stacks.cdc.gov/view/cdc/32267/cdc_32267_DS1.pdf
File Type:

Supporting Files

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