In Vivo Imaging of Hedgehog Pathway Activation with a Nuclear Fluorescent Reporter

Details

• Alternative Title:
  PLoS One
• Personal Author:
  Mich, John K. ; Payumo, Alexander Y. ; Rack, Paul G. ; Chen, James K.
• Description:
  The Hedgehog (Hh) pathway is essential for embryonic development and tissue regeneration, and its dysregulation can lead to birth defects and tumorigenesis. Understanding how this signaling mechanism contributes to these processes would benefit from an ability to visualize Hedgehog pathway activity in live organisms, in real time, and with single-cell resolution. We report here the generation of transgenic zebrafish lines that express nuclear-localized mCherry fluorescent protein in a Gli transcription factor-dependent manner. As demonstrated by chemical and genetic perturbations, these lines faithfully report Hedgehog pathway state in individual cells and with high detection sensitivity. They will be valuable tools for studying dynamic Gli-dependent processes in vertebrates and for identifying new chemical and genetic regulators of the Hh pathway.
• Subjects:
  Animal Fins Animals Animals, Genetically Modified Cell Nucleus Green Fluorescent Proteins Hedgehog Proteins Luminescent Proteins Microscopy, Fluorescence Mutation Reproducibility Of Results Signal Transduction Somites Time-Lapse Imaging Zebrafish Zebrafish Proteins

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• Source:
  PLoS One. 2014; 9(7).
• Pubmed ID:
  25068273
• Pubmed Central ID:
  PMC4113417
• Document Type:
  Journal Article
• Funding:
  DP1 HD075622/DP/NCCDPHP CDC HHS/United States ; P50 GM107615/GM/NIGMS NIH HHS/United States
• Volume:
  9
• Issue:
  7
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:a86ae709b2e78a1a9e45aa2ba84d42f152e38e5055e2d80e8f744fd1da429d7e
• Download URL:
  https://stacks.cdc.gov/view/cdc/32191/cdc_32191_DS1.pdf
• File Type:
  [PDF - 1.15 MB ]