Welcome to CDC stacks | In Vivo Imaging of Hedgehog Pathway Activation with a Nuclear Fluorescent Reporter - 32191 | CDC Public Access
Stacks Logo
Advanced Search
Select up to three search categories and corresponding keywords using the fields to the right. Refer to the Help section for more detailed instructions.
 
 
Help
Clear All Simple Search
Advanced Search
In Vivo Imaging of Hedgehog Pathway Activation with a Nuclear Fluorescent Reporter
Filetype[PDF-1.15 MB]


Details:
  • Pubmed ID:
    25068273
  • Pubmed Central ID:
    PMC4113417
  • Description:
    The Hedgehog (Hh) pathway is essential for embryonic development and tissue regeneration, and its dysregulation can lead to birth defects and tumorigenesis. Understanding how this signaling mechanism contributes to these processes would benefit from an ability to visualize Hedgehog pathway activity in live organisms, in real time, and with single-cell resolution. We report here the generation of transgenic zebrafish lines that express nuclear-localized mCherry fluorescent protein in a Gli transcription factor-dependent manner. As demonstrated by chemical and genetic perturbations, these lines faithfully report Hedgehog pathway state in individual cells and with high detection sensitivity. They will be valuable tools for studying dynamic Gli-dependent processes in vertebrates and for identifying new chemical and genetic regulators of the Hh pathway.

  • Document Type:
  • Collection(s):
  • Funding:
    DP1 HD075622/DP/NCCDPHP CDC HHS/United States
    P50 GM107615/GM/NIGMS NIH HHS/United States
No Related Documents.
You May Also Like: