Details:

Alternative Title:
PLoS One
Publisher's site:
http://dx.doi.org/10.1371/journal.pone.0102694
Personal Author:
Marshall, John M. ; Hay, Bruce A. ;
Marshall, John M. ; Hay, Bruce A. ; Less -
Description:
Gene drive systems provide novel opportunities for insect population suppression by driving genes that confer a fitness cost into pest or disease vector populations; however regulatory issues arise when genes are capable of spreading across international borders. Gene drive systems displaying threshold properties provide a solution since they can be confined to local populations and eliminated through dilution with wild-types. We propose a novel, threshold-dependent gene drive system, Medusa, capable of inducing a local and reversible population crash. Medusa consists of four components--two on the X chromosome, and two on the Y chromosome. A maternally-expressed, X-linked toxin and a zygotically-expressed, Y-linked antidote results in suppression of the female population and selection for the presence of the transgene-bearing Y because only male offspring of Medusa-bearing females are protected from the effects of the toxin. At the same time, the combination of a zygotically-expressed, Y-linked toxin and a zygotically-expressed, X-linked antidote selects for the transgene-bearing X in the presence of the transgene-bearing Y. Together these chromosomes create a balanced lethal system that spreads while selecting against females when present above a certain threshold frequency. Simple population dynamic models show that an all-male release of Medusa males, carried out over six generations, is expected to induce a population crash within 12 generations for modest release sizes on the order of the wild population size. Re-invasion of non-transgenic insects into a suppressed population can result in a population rebound; however this can be prevented through regular releases of modest numbers of Medusa males. Finally, we outline how Medusa could be engineered with currently available molecular tools.
Subject:
[+]
Algorithms
Animals
Animals, Genetically Modified
Antidotes
Chromosomes, Insect
Female
Genetic Engineering
Genetics, Population
Inheritance Patterns
Insect Control
Male
Models, Genetic
Time Factors
Toxins, Biological
Transgenes
X Chromosome
Y Chromosome

Source:
PLoS One. 2014 ; 9(7).;
Pubmed ID:
25054803
Pubmed Central ID:
PMC4108329
Document Type:
Journal Article ;
Funding:
DP1GM1053A/DP/NCCDPHP CDC HHS/United States ; Medical Research Council/United Kingdom ;
DP1GM1053A/DP/NCCDPHP CDC HHS/United States ; Medical Research Council/United Kingdom ; Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:94b44b2e7cf6825ae69170d8f7a2c647a8f20dc696c215289b6488bd7bff8cad
File Type:

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