Differential targeting of feline photoreceptors by recombinant adeno-associated viral vectors: implications for preclinical gene therapy trials

Details

• Alternative Title:
  Gene Ther
• Personal Author:
  Minella, AL ; Mowat, FM ; Willett, KL ; Sledge, D ; Bartoe, JT ; Bennett, J ; Petersen-Jones, SM
• Description:
  The cat is emerging as a promising large animal model for preclinical testing of retinal dystrophy therapies, for example, by gene therapy. However, there is a paucity of studies investigating viral vector gene transfer to the feline retina. We therefore sought to study the tropism of recombinant adeno-associated viral (rAAV) vectors for the feline outer retina. We delivered four rAAV serotypes: rAAV2/2, rAAV2/5, rAAV2/8 and rAAV2/9, each expressing green fluorescent protein (GFP) under the control of a cytomegalovirus promoter, to the subretinal space in cats and, for comparison, mice. Cats were monitored for gene expression by in vivo imaging and cellular tropism was determined using immunohistochemistry. In cats, rAAV2/2, rAAV2/8 and rAAV2/9 vectors induced faster and stronger GFP expression than rAAV2/5 and all vectors transduced the retinal pigment epithelium (RPE) and photoreceptors. Unlike in mice, cone photoreceptors in the cat retina were more efficiently transduced than rod photoreceptors. In mice, rAAV2/2 only transduced the RPE whereas the other vectors also transduced rods and cones. These results highlight species differences in cellular tropism of rAAV vectors in the outer retina. We conclude that rAAV serotypes are suitable for use for retinal gene therapy in feline models, particularly when cone photoreceptors are the target cell.
• Subjects:
  Animals Article Cats Dependovirus Female Genetic Therapy Genetic Vectors Green Fluorescent Proteins Injections, Intraocular Male Mice Retinal Cone Photoreceptor Cells Retinal Rod Photoreceptor Cells Transduction, Genetic Viral Tropism

  More +
• Source:
  Gene Ther. 2014; 21(10):913-920
• Pubmed ID:
  25056608
• Pubmed Central ID:
  PMC4487421
• Document Type:
  Journal Article
• Funding:
  1R24EY019861/EY/NEI NIH HHSUnited States/ ; T32 OD011167/OD/NIH HHSUnited States/ ; R24 EY019861/EY/NEI NIH HHSUnited States/ ; DP1 EY023177/EY/NEI NIH HHSUnited States/ ; P30 EY001583/EY/NEI NIH HHSUnited States/ ; T32OD011167/OD/NIH HHSUnited States/ ; 8DP1EY023177/DP/NCCDPHP CDC HHSUnited States/
• Volume:
  21
• Issue:
  10
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:8b2a0e3832ec3c31b1bd3dd1c7620a69dee1f8504f30a1e970daccb311f710cc
• Download URL:
  https://stacks.cdc.gov/view/cdc/31940/cdc_31940_DS1.pdf
• File Type:
  [PDF - 2.09 MB ]