Two-Component Direct Fluorescent-Antibody Assay for Rapid Identification of Bacillus anthracis

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  De, Barun K. ; Bragg, Sandra L. ; Sanden, Gary N. ; Wilson, Kathy E. ; Diem, Lois A. ; Marston, Chung K. ; Hoffmaster, Alex R. ; Barnet, Gwen A. ; Weyant, Robbin S. ; Abshire, Teresa G. ; Ezzell, John W. ; Popovic, Tanja
• Description:
  A two-component direct fluorescent-antibody (DFA) assay, using fluorescein-labeled monoclonal antibodies specific to the Bacillus anthracis cell wall (CW-DFA) and capsule (CAP-DFA) antigens, was evaluated and validated for rapid identification of B. anthracis. We analyzed 230 B. anthracis isolates; 228 and 229 were positive by CW-DFA and CAP-DFA assays, respectively. We also tested 56 non-B. anthracis strains; 10 B. cereus and 2 B. thuringiensis were positive by the CW-DFA assay, and 1 B. megaterium strain was positive by CAP-DFA. Analysis of the combined DFA results identified 227 of 230 B. anthracis isolates; all 56 strains of the other Bacillus spp. were negative. Both DFA assays tested positive on 14 of 26 aging clinical specimens from the 2001 anthrax outbreak investigation. The two-component DFA assay is a sensitive, specific, and rapid confirmatory test for B. anthracis in cultures and may be useful directly on clinical specimens.
• Subjects:
  Animals Anthrax Antibodies, Monoclonal Antigens, Bacterial Bacterial Capsules Cattle Cell Wall Dispatch Environmental Monitoring Fluorescent Antibody Technique Humans Sensitivity And Specificity

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• Source:
  Emerg Infect Dis. 8(10):1060-1065.
• Document Type:
  Journal Article
• Volume:
  8
• Issue:
  10
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:f062ae88504568b46007c22782527767dbf19775fb2733c94c1de0a94fb99afe
• Download URL:
  https://stacks.cdc.gov/view/cdc/3116/cdc_3116_DS1.pdf
• File Type:
  [PDF - 727.20 KB ]