Two-Component Direct Fluorescent-Antibody Assay for Rapid Identification of Bacillus anthracis

De, Barun K.; Bragg, Sandra L.; Sanden, Gary N.; Wilson, Kathy E.; Diem, Lois A.; Marston, Chung K.; Hoffmaster, Alex R.; Barnet, Gwen A.; Weyant, Robbin S.; Abshire, Teresa G.; Ezzell, John W.; Popovic, Tanja

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Two-Component Direct Fluorescent-Antibody Assay for Rapid Identification of Bacillus anthracis

Oct 2002
By De, Barun K. ; Bragg, Sandra L. ; Sanden, Gary N. ; ...
Source: Emerg Infect Dis. 8(10):1060-1065.

[PDF-727.20 KB]

English

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Details:

Alternative Title:

Emerg Infect Dis
Personal Author:

De, Barun K. ; Bragg, Sandra L. ; Sanden, Gary N. ; Wilson, Kathy E. ; Diem, Lois A. ; ... More +

De, Barun K. ; Bragg, Sandra L. ; Sanden, Gary N. ; Wilson, Kathy E. ; Diem, Lois A. ; Marston, Chung K. ; Hoffmaster, Alex R. ; Barnet, Gwen A. ; Weyant, Robbin S. ; Abshire, Teresa G. ; Ezzell, John W. ; Popovic, Tanja Less -
Description:

A two-component direct fluorescent-antibody (DFA) assay, using fluorescein-labeled monoclonal antibodies specific to the Bacillus anthracis cell wall (CW-DFA) and capsule (CAP-DFA) antigens, was evaluated and validated for rapid identification of B. anthracis. We analyzed 230 B. anthracis isolates; 228 and 229 were positive by CW-DFA and CAP-DFA assays, respectively. We also tested 56 non-B. anthracis strains; 10 B. cereus and 2 B. thuringiensis were positive by the CW-DFA assay, and 1 B. megaterium strain was positive by CAP-DFA. Analysis of the combined DFA results identified 227 of 230 B. anthracis isolates; all 56 strains of the other Bacillus spp. were negative. Both DFA assays tested positive on 14 of 26 aging clinical specimens from the 2001 anthrax outbreak investigation. The two-component DFA assay is a sensitive, specific, and rapid confirmatory test for B. anthracis in cultures and may be useful directly on clinical specimens.
Subjects:

[+]

Animals Anthrax Antibodies, Monoclonal Antigens, Bacterial Bacterial Capsules Cattle Cell Wall Dispatch Environmental Monitoring Fluorescent Antibody Technique Humans Sensitivity And Specificity
Source:

Emerg Infect Dis. 8(10):1060-1065.
Document Type:

Journal Article
Collection(s):

Emerging Infectious Diseases
Main Document Checksum:

[+]

urn:sha256:f062ae88504568b46007c22782527767dbf19775fb2733c94c1de0a94fb99afe
Download URL:

https://stacks.cdc.gov/view/cdc/3116/cdc_3116_DS1.pdf
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