National Tuberculosis Genotyping and Surveillance Network: Design and Methods

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Crawford, Jack T. ; Braden, Christopher R. ; Schable, Barbara A. ; Onorato, Ida M.
• Description:
  The National Tuberculosis Genotyping and Surveillance Network was established in 1996 to perform a 5-year, prospective study of the usefulness of genotyping Mycobacterium tuberculosis isolates to tuberculosis control programs. Seven sentinel sites identified all new cases of tuberculosis, collected information on patients and contacts, and obtained patient isolates. Seven genotyping laboratories performed DNA fingerprinting analysis by the international standard IS6110 method. BioImage Whole Band Analyzer software was used to analyze patterns, and distinct patterns were assigned unique designations. Isolates with six or fewer bands on IS6110 patterns were also spoligotyped. Patient data and genotyping designations were entered in a relational database and merged with selected variables from the national surveillance database. In two related databases, we compiled the results of routine contact investigations and the results of investigations of the relationships of patients who had isolates with matching genotypes. We describe the methods used in the study.
• Subjects:
  Bacterial Typing Techniques Communicable Disease Control Databases, Factual Dispatch Genotype Humans Sentinel Surveillance Tuberculosis
• Source:
  Emerg Infect Dis. 8(11):1192-1196.
• Document Type:
  Journal Article
• Name as Subject:
  Centers for Disease Control and Prevention (U.S.) ; Centers for Disease Control and Prevention (U.S.)
• Place as Subject:
  United States
• Location:
  North America
• Volume:
  8
• Issue:
  11
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:d88453aa0da1951a864883562926967edff359b7da03be9cb441480285ba63b0
• Download URL:
  https://stacks.cdc.gov/view/cdc/3099/cdc_3099_DS1.pdf
• File Type:
  [PDF - 267.56 KB ]