Details:

Alternative Title:
Genome Biol Evol
Publisher's site:
http://dx.doi.org/10.1093/gbe/evu079
Personal Author:
Hoffmann, Maria; Zhao, Shaohua; Pettengill, James; Luo, Yan; Monday, Steven R.; ... More +
Hoffmann, Maria; Zhao, Shaohua; Pettengill, James; Luo, Yan; Monday, Steven R.; Abbott, Jason; Ayers, Sherry L.; Cinar, Hediye N.; Muruvanda, Tim; Li, Cong; Allard, Marc W.; Whichard, Jean; Meng, Jianghong; Brown, Eric W.; McDermott, Patrick F.; Less -
Description:
Salmonella enterica subsp. enterica serovar Heidelberg (S. Heidelberg) is one of the top serovars causing human salmonellosis. Recently, an antibiotic-resistant strain of this serovar was implicated in a large 2011 multistate outbreak resulting from consumption of contaminated ground turkey that involved 136 confirmed cases, with one death. In this study, we assessed the evolutionary diversity of 44 S. Heidelberg isolates using whole-genome sequencing (WGS) generated by the 454 GS FLX (Roche) platform. The isolates, including 30 with nearly indistinguishable (one band difference) Xbal pulsed-field gel electrophoresis patterns (JF6X01.0032, JF6X01.0058), were collected from various sources between 1982 and 2011 and included nine isolates associated with the 2011 outbreak. Additionally, we determined the complete sequence for the chromosome and three plasmids from a clinical isolate associated with the 2011 outbreak using the Pacific Biosciences (PacBio) system. Using single-nucleotide polymorphism (SNP) analyses, we were able to distinguish highly clonal isolates, including strains isolated at different times in the same year. The isolates from the recent 2011 outbreak clustered together with a mean SNP variation of only 17 SNPs. The S. Heidelberg isolates carried a variety of phages, such as prophage P22, P4, lambda-like prophage Gifsy-2, and the P2-like phage which carries the sopE1 gene, virulence genes including 62 pathogenicity, and 13 fimbrial markers and resistance plasmids of the incompatibility (Inc)I1, IncA/C, and IncHI2 groups. Twenty-one strains contained an IncX plasmid carrying a type IV secretion system. On the basis of the recent and historical isolates used in this study, our results demonstrated that, in addition to providing detailed genetic information for the isolates, WGS can identify SNP targets that can be utilized for differentiating highly clonal S. Heidelberg isolates.
Subject:
[+]
Antimicrobial Resistance
Outbreak
Plasmid
Research Article
SNP Analysis
Trace-back

Source:
Genome Biol Evol. 2014; 6(5):1046-1068.;
Document Type:
Journal Article;
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:3f53e18cbb8650509483cdc9afe8dd371b035bab5d2bf83dc75eec6848e1ffc6
File Type:

Supporting Files

• 	evu079f4p.jpg	jpeg
  	evu079f5p.gif	gif
  	evu079f5p.jpg	jpeg
  	evu079f6p.gif	gif
  	evu079f6p.jpg	jpeg
  	evu079f7p.gif	gif
  	evu079f7p.jpg	jpeg
  	evu079f8p.gif	gif
  	evu079f8p.jpg	jpeg
  	evu079.nxml	txt
  	license.txt	txt
  	supp_6_5_1046__index.html	htm
  	supp_evu079_Supp_Figure_S1.tif	tiff
  	supp_evu079_Supp_Figure_S2.tif	tiff
  	supp_evu079_Supp_Figure_S3.tif	tiff
  	supp_evu079_Supp_Figure_S4.tif	tiff
  	supp_evu079_Supp_Table_S1.xls	xls
  	supp_evu079_Supp_Table_S2.xls	xls
  	evu079f1p.gif	gif
  	evu079f1p.jpg	jpeg
  	evu079f2p.gif	gif
  	evu079f2p.jpg	jpeg
  	evu079f3p.gif	gif
  	evu079f3p.jpg	jpeg
  	evu079f4p.gif	gif

More +