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CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling
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  1. Applications of CRISPR-Cas systems in neuroscience
    Personal Author:
    Heidenreich, Matthias; Zhang, Feng;
    Published Date:
    Dec 10 2015
    Source:
    Nat Rev Neurosci. 17(1):36-44.
    Description:
    Genome-editing tools, and in particular those based on CRISPR-Cas (clustered regularly interspaced short palindromic repeat (CRISPR)-CRISPR-associated protein) systems, are accelerating the pace of biological research and enabling targeted genetic in...
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  2. Orthogonal gene knock out and activation with a catalytically active Cas9 nuclease
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    Dahlman, James E.; Abudayyeh, Omar O.; Joung, Julia; Gootenberg, Jonathan S.; Zhang, Feng; Konermann, Silvana;
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    Nov 2015
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    Nat Biotechnol. 33(11):1159-1161.
    Description:
    We have developed a CRISPR-based method that uses catalytically active Cas9 and distinct single guide (sgRNA) constructs to knock out and activate different genes in the same cell. These sgRNAs, with 14- to 15-bp target sequences and MS2 binding loop...
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  3. In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9
    Personal Author:
    Swiech, Lukasz; Heidenreich, Matthias; Banerjee, Abhishek; Habib, Naomi; Li, Yinqing; Trombetta, John; Sur, Mriganka; Zhang, Feng;
    Published Date:
    Oct 19 2014
    Source:
    Nat Biotechnol. 2014; 33(1):102-106.
    Description:
    Probing gene function in the mammalian brain can be greatly assisted with methods to manipulate the genome of neurons in vivo. The clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated endonuclease (Cas)9 from Streptococcus ...
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  4. High-throughput functional genomics using CRISPR-Cas9
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    Shalem, Ophir; Sanjana, Neville E.; Zhang, Feng;
    Published Date:
    Apr 09 2015
    Source:
    Nat Rev Genet. 2015; 16(5):299-311.
    Description:
    Forward genetic screens are powerful tools for the discovery and functional annotation of genetic elements. Recently, the RNA-guided CRISPR (clustered regularly interspaced short palindromic repeat)-associated Cas9 nuclease has been combined with gen...
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  5. Development and Applications of CRISPR-Cas9 for Genome Engineering
    Personal Author:
    Hsu, Patrick D.; Lander, Eric S.; Zhang, Feng;
    Published Date:
    Jun 5 2014
    Source:
    Cell. 2014; 157(6):1262-1278.
    Description:
    Recent advances in genome engineering technologies based on the CRISPR-associated RNA-guided endonuclease Cas9 are enabling the systematic interrogation of mammalian genome function. Analogous to the search function in modern word processors, Cas9 ca...
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