Short Report: Olfactory Ensheathing Cells Promote Differentiation of Neural Stem Cells and Robust Neurite Extension

Details

• Alternative Title:
  Stem Cell Rev
• Personal Author:
  Sethi, Rosh ; Sethi, Roshan ; Redmond, Andy ; Lavik, Erin
• Description:
  Aims
  
  The goal of this study was to gain insight into the signaling between olfactory ensheathing cells (OECs) and neural stem cells (NSCs). We sought to understand the impact of OECs on NSC differentiation and neurite extension and to begin to elucidate the factors involved in these interactions to provide new targets for therapeutic interventions.
  
  Materials and Methods
  
  We utilized lines of OECs that have been extremely well characterized in vitro and in vivo along with well studied NSCs in gels to determine the impact of the coculture in three dimensions. To further elucidate the signaling, we used conditioned media from the OECs as well as fractioned components on NSCs to determine the molecular weight range of the soluble factors that was most responsible for the NSC behavior.
  
  Results
  
  We found that the coculture of NSCs and OECs led to robust NSC differentiation and extremely long neural processes not usually seen with NSCs in three dimensional gels in vitro. Through culture of NSCs with fractioned OEC media, we determined that molecules larger than 30 kDa have the greatest impact on the NSC behavior.
  
  Conclusions
  
  Overall, our findings suggest that cocultures of NSCs and OECs may be a novel combination therapy for neural injuries including spinal cord injury (SCI). Furthermore, we have identified a class of molecules which plays a substantial role in the behavior that provides new targets for investigating pharmacological therapies.
  
  
• Subjects:
  Animals Cell Differentiation Coculture Techniques Humans Neural Stem Cells Neurites Neurons Olfactory Cortex
• Keywords:
  CNS Injury Neural Progenitor Olfactory Ensheathing Glia Paracrine Signaling Scaffold Tissue Engineering
• Source:
  Stem Cell Rev. 10(6):772-785
• Pubmed ID:
  24996386
• Pubmed Central ID:
  PMC4238386
• Document Type:
  Journal Article
• Funding:
  DP2 OD007338/OD/NIH HHSUnited States/ ; DP20D007338/DP/NCCDPHP CDC HHSUnited States/
• Volume:
  10
• Issue:
  6
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha-512:a52d30787cfd0e706c1628391137c471ad3e187734e774ca43f58d30397a711e7e808b0d7bef2ecebe367f78d176a5c44bf84516bcefc36a392170fcd7cfcb11
• Download URL:
  https://stacks.cdc.gov/view/cdc/30181/cdc_30181_DS1.pdf
• File Type:
  [PDF - 1.62 MB ]