Details:

Alternative Title:
Stem Cell Rev
Personal Author:
Sethi, Rosh ; Sethi, Roshan ; Redmond, Andy ; Lavik, Erin
Sethi, Rosh ; Sethi, Roshan ; Redmond, Andy ; Lavik, Erin Less -
Description:
Aims

The goal of this study was to gain insight into the signaling between olfactory ensheathing cells (OECs) and neural stem cells (NSCs). We sought to understand the impact of OECs on NSC differentiation and neurite extension and to begin to elucidate the factors involved in these interactions to provide new targets for therapeutic interventions.

Materials and Methods

We utilized lines of OECs that have been extremely well characterized in vitro and in vivo along with well studied NSCs in gels to determine the impact of the coculture in three dimensions. To further elucidate the signaling, we used conditioned media from the OECs as well as fractioned components on NSCs to determine the molecular weight range of the soluble factors that was most responsible for the NSC behavior.

Results

We found that the coculture of NSCs and OECs led to robust NSC differentiation and extremely long neural processes not usually seen with NSCs in three dimensional gels in vitro. Through culture of NSCs with fractioned OEC media, we determined that molecules larger than 30 kDa have the greatest impact on the NSC behavior.

Conclusions

Overall, our findings suggest that cocultures of NSCs and OECs may be a novel combination therapy for neural injuries including spinal cord injury (SCI). Furthermore, we have identified a class of molecules which plays a substantial role in the behavior that provides new targets for investigating pharmacological therapies.


Subject:
[+]
Animals
Article
Cell Differentiation
CNS Injury
Coculture Techniques
Humans
Neural Progenitor
Neural Stem Cells
Neurites
Neurons
Olfactory Cortex
Olfactory Ensheathing Glia
Paracrine Signaling
Scaffold
Tissue Engineering

Source:
Stem Cell Rev. 10(6):772-785
Pubmed ID:
24996386
Pubmed Central ID:
PMC4238386
Document Type:
Journal Article
Funding:
DP2 OD007338/OD/NIH HHS/United States ; DP20D007338/DP/NCCDPHP CDC HHS/United States
DP2 OD007338/OD/NIH HHS/United States ; DP20D007338/DP/NCCDPHP CDC HHS/United States Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:f2b1e5734d17bbd01aef5ad099e1179a399af323dc2a25a07f7e51251923848c
File Type:

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