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Near-infrared Optical-resolution Photoacoustic Microscopy
  • Published Date:
    Sep 1 2014
  • Source:
    Opt Lett. 2014; 39(17):5192-5195.
Filetype[PDF - 738.20 KB]


Details:
  • Pubmed ID:
    25166107
  • Pubmed Central ID:
    PMC4161671
  • Funding:
    DP1 EB016986/DP/NCCDPHP CDC HHS/United States
    DP1 EB016986/EB/NIBIB NIH HHS/United States
    R01 CA159959/CA/NCI NIH HHS/United States
    R01 CA159959/CA/NCI NIH HHS/United States
    R01 CA186567/CA/NCI NIH HHS/United States
    R01 CA186567/CA/NCI NIH HHS/United States
  • Document Type:
  • Collection(s):
  • Description:
    Compared with visible light (380-700 nm), near-infrared light (700-1400 nm) undergoes weaker optical attenuation in biological tissue; thus, it can penetrate deeper. Herein, we demonstrate near-infrared optical-resolution photoacoustic microscopy (NIR-OR-PAM) with 1046 nm illumination. A penetration depth of 3.2 mm was achieved in chicken breast tissue ex vivo using optical fluence within the American National Standards Institute (ANSI) limit (100  mJ/cm2). Beyond ∼0.6  mm deep in chicken breast tissue, NIR-OR-PAM has shown finer resolution than the visible counterpart with 570 nm illumination. The deep imaging capability of NIR-OR-PAM was validated in both a mouse ear and a mouse brain. NIR-OR-PAM of possible lipid contrast was explored as well.