Near-infrared Optical-resolution Photoacoustic Microscopy

Details

• Alternative Title:
  Opt Lett
• Personal Author:
  Hai, Pengfei ; Yao, Junjie ; Maslov, Konstantin I. ; Zhou, Yong ; Wang, Lihong V.
• Description:
  Compared with visible light (380-700 nm), near-infrared light (700-1400 nm) undergoes weaker optical attenuation in biological tissue; thus, it can penetrate deeper. Herein, we demonstrate near-infrared optical-resolution photoacoustic microscopy (NIR-OR-PAM) with 1046 nm illumination. A penetration depth of 3.2 mm was achieved in chicken breast tissue ex vivo using optical fluence within the American National Standards Institute (ANSI) limit (100  mJ/cm2). Beyond ∼0.6  mm deep in chicken breast tissue, NIR-OR-PAM has shown finer resolution than the visible counterpart with 570 nm illumination. The deep imaging capability of NIR-OR-PAM was validated in both a mouse ear and a mouse brain. NIR-OR-PAM of possible lipid contrast was explored as well.
• Subjects:
  Animals Brain Chickens Diagnostic Imaging Ear Humans Infrared Rays Lasers, Solid-State Mice Microscopy Optical Phenomena Photoacoustic Techniques

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• Source:
  Opt Lett. 2014; 39(17):5192-5195
• Pubmed ID:
  25166107
• Pubmed Central ID:
  PMC4161671
• Document Type:
  Journal Article
• Funding:
  DP1 EB016986/EB/NIBIB NIH HHSUnited States/ ; R01 CA159959/CA/NCI NIH HHSUnited States/ ; R01 CA186567/CA/NCI NIH HHSUnited States/ ; DP1 EB016986/DP/NCCDPHP CDC HHSUnited States/
• Volume:
  39
• Issue:
  17
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:c2ca5e1a31fd7aa3a24506581b1fa2d6399e67ad36617e9ae8e8d5ca58cf38bb
• Download URL:
  https://stacks.cdc.gov/view/cdc/30114/cdc_30114_DS1.pdf
• File Type:
  [PDF - 738.20 KB ]