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In Vivo Time-gated Fluorescence Imaging with Biodegradable Luminescent Porous Silicon Nanoparticles
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  • Description:
    Fluorescence imaging is one of the most versatile and widely used visualization methods in biomedical research. However, tissue autofluorescence is a major obstacle confounding interpretation of in vivo fluorescence images. The unusually long emission lifetime (5-13 μs) of photoluminescent porous silicon nanoparticles can allow the time-gated imaging of tissues in vivo, completely eliminating shorter-lived (<10 ns) emission signals from organic chromophores or tissue autofluorescence. Here using a conventional animal imaging system not optimized for such long-lived excited states, we demonstrate improvement of signal to background contrast ratio by >50-fold in vitro and by >20-fold in vivo when imaging porous silicon nanoparticles. Time-gated imaging of porous silicon nanoparticles accumulated in a human ovarian cancer xenograft following intravenous injection is demonstrated in a live mouse. The potential for multiplexing of images in the time domain by using separate porous silicon nanoparticles engineered with different excited state lifetimes is discussed.

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    1DP20D006499-01/DP/NCCDPHP CDC HHS/United States
    5-R01-CA124427/CA/NCI NIH HHS/United States
    5R01EB015498/EB/NIBIB NIH HHS/United States
    CA095298/CA/NCI NIH HHS/United States
    CA152185/CA/NCI NIH HHS/United States
    DP2 OD006499/OD/NIH HHS/United States
    HHMI56005681/Howard Hughes Medical Institute/United States
    P50 CA128346/CA/NCI NIH HHS/United States
    P50CA128346/CA/NCI NIH HHS/United States
    R01 CA095298/CA/NCI NIH HHS/United States
    R01 CA124427/CA/NCI NIH HHS/United States
    R01 CA152185/CA/NCI NIH HHS/United States
    R01 EB015498/EB/NIBIB NIH HHS/United States
    U54 CA119335/CA/NCI NIH HHS/United States
    U54-CA 119335/CA/NCI NIH HHS/United States
    Howard Hughes Medical Institute/United States
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