In Vivo Time-gated Fluorescence Imaging with Biodegradable Luminescent Porous Silicon Nanoparticles

Details

• Alternative Title:
  Nat Commun
• Personal Author:
  Gu, Luo ; Hall, David J. ; Qin, Zhengtao ; Anglin, Emily ; Joo, Jinmyoung ; Mooney, David J. ; Howell, Stephen B. ; Sailor, Michael J.
• Description:
  Fluorescence imaging is one of the most versatile and widely used visualization methods in biomedical research. However, tissue autofluorescence is a major obstacle confounding interpretation of in vivo fluorescence images. The unusually long emission lifetime (5-13 μs) of photoluminescent porous silicon nanoparticles can allow the time-gated imaging of tissues in vivo, completely eliminating shorter-lived (<10 ns) emission signals from organic chromophores or tissue autofluorescence. Here using a conventional animal imaging system not optimized for such long-lived excited states, we demonstrate improvement of signal to background contrast ratio by >50-fold in vitro and by >20-fold in vivo when imaging porous silicon nanoparticles. Time-gated imaging of porous silicon nanoparticles accumulated in a human ovarian cancer xenograft following intravenous injection is demonstrated in a live mouse. The potential for multiplexing of images in the time domain by using separate porous silicon nanoparticles engineered with different excited state lifetimes is discussed.
• Subjects:
  Animals Article Female Humans Luminescent Agents Mice Nanoparticles Neoplasm Transplantation Optical Imaging Ovarian Neoplasms Silicon Xenograft Model Antitumor Assays

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• Source:
  Nat Commun. 4:2326
• Pubmed ID:
  23933660
• Pubmed Central ID:
  PMC4154512
• Document Type:
  Journal Article
• Funding:
  CA095298/CA/NCI NIH HHS/United States ; U54 CA119335/CA/NCI NIH HHS/United States ; U54-CA 119335/CA/NCI NIH HHS/United States ; R01 CA152185/CA/NCI NIH HHS/United States ; 5R01EB015498/EB/NIBIB NIH HHS/United States ; CA152185/CA/NCI NIH HHS/United States ; R01 CA095298/CA/NCI NIH HHS/United States ; DP2 OD006499/OD/NIH HHS/United States ; 1DP20D006499-01/DP/NCCDPHP CDC HHS/United States ; HHMI56005681/Howard Hughes Medical Institute/United States ; P50CA128346/CA/NCI NIH HHS/United States ; Howard Hughes Medical Institute/United States ; R01 EB015498/EB/NIBIB NIH HHS/United States ; 5-R01-CA124427/CA/NCI NIH HHS/United States ; P50 CA128346/CA/NCI NIH HHS/United States ; R01 CA124427/CA/NCI NIH HHS/United States
• Volume:
  4
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:a3bdc1fc9bc21ad9c570d11994c868dbf5dbdada0fc41378d6b60fe09aed089c
• Download URL:
  https://stacks.cdc.gov/view/cdc/30102/cdc_30102_DS1.pdf
• File Type:
  [PDF - 498.50 KB ]