In Vivo Time-gated Fluorescence Imaging with Biodegradable Luminescent Porous Silicon Nanoparticles

Gu, Luo; Hall, David J.; Qin, Zhengtao; Anglin, Emily; Joo, Jinmyoung; Mooney, David J.; Howell, Stephen B.; Sailor, Michael J.

doi:10.1038/ncomms3326

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In Vivo Time-gated Fluorescence Imaging with Biodegradable Luminescent Porous Silicon Nanoparticles

Published Date:

2013

Publisher's site:

http://dx.doi.org/10.1038/ncomms3326 New Site Icon

Source:

Nat Commun. 4:2326.

[PDF-498.50 KB]

Details:

Personal Authors:

Gu, Luo ; Hall, David J. ; Qin, Zhengtao ; Anglin, Emily ; Joo, Jinmyoung ; ... More ▼

Keywords:

[+]

Pubmed ID:

23933660

Pubmed Central ID:

PMC4154512

Description:

Fluorescence imaging is one of the most versatile and widely used visualization methods in biomedical research. However, tissue autofluorescence is a major obstacle confounding interpretation of in vivo fluorescence images. The unusually long emission lifetime (5-13 μs) of photoluminescent porous silicon nanoparticles can allow the time-gated imaging of tissues in vivo, completely eliminating shorter-lived (<10 ns) emission signals from organic chromophores or tissue autofluorescence. Here using a conventional animal imaging system not optimized for such long-lived excited states, we demonstrate improvement of signal to background contrast ratio by >50-fold in vitro and by >20-fold in vivo when imaging porous silicon nanoparticles. Time-gated imaging of porous silicon nanoparticles accumulated in a human ovarian cancer xenograft following intravenous injection is demonstrated in a live mouse. The potential for multiplexing of images in the time domain by using separate porous silicon nanoparticles engineered with different excited state lifetimes is discussed.

Document Type:

Journal Article

Collection(s):

CDC Public Access

Funding:

Main Document Checksum:

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Supporting Files:

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