Details:

Alternative Title:
Biofabrication
Personal Author:
Szymanski, John M ; Feinberg, Adam W
Szymanski, John M ; Feinberg, Adam W Less -
Description:
Natural biopolymers such as alginate have become important materials for a variety of biotechnology applications including drug delivery, cell encapsulation and tissue engineering. This expanding use has spurred the development of new approaches to engineer these materials at the nano- and microscales to better control cell interactions. Here we describe a method to fabricate freestanding alginate-based microfibers and microstructures with tunable geometries down to approximately 3 µm. To do this, a polydimethylsiloxane stamp is used to micromold alginate or alginate-fibrin blends onto a sacrificial layer of thermally-sensitive poly(N-isopropylacrylamide) (PIPAAm). A warm calcium chloride solution is then used to crosslink the alginate and, upon cooling below the lower critical solution temperature (~32 °C), the PIPAAm layer dissolves and releases the alginate or alginate-fibrin as freestanding microfibers and microstructures. Proof-of-concept experiments demonstrate that C2C12 myoblasts seeded onto the alginate-fibrin microfibers polarize along the fiber length forming interconnected cell strands. Thus, we have developed the ability to engineer alginate-based microstructured materials that can selectively bind cells and direct cellular assembly.
Subjects:
[+]
Alginates Animals Article Biocompatible Materials Biopolymers Cell Adhesion Cell Line Dimethylpolysiloxanes Fibrin Glucuronic Acid Hexuronic Acids Mice Tissue Engineering Tissue Scaffolds
Source:
Biofabrication. 6(2):024104.
Pubmed ID:
24695323
Pubmed Central ID:
PMC4130459
Document Type:
Journal Article
Funding:
1DP2HL117750/DP/NCCDPHP CDC HHS/United States ; 2T32EB003392/EB/NIBIB NIH HHS/United States ; DP2 HL117750/HL/NHLBI NIH HHS/United States ; T32 EB003392/EB/NIBIB NIH HHS/United States
1DP2HL117750/DP/NCCDPHP CDC HHS/United States ; 2T32EB003392/EB/NIBIB NIH HHS/United States ; DP2 HL117750/HL/NHLBI NIH HHS/United States ; T32 EB003392/EB/NIBIB NIH HHS/United States Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:5f06ee5bb9f6bf24c6f637562ec34a957324db9707c65f4b5d35b1216ffa6dfc
Download URL:
https://stacks.cdc.gov/view/cdc/30079/cdc_30079_DS1.pdf
File Type:

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