Details:

Alternative Title:
Small
Personal Author:
Andón, Fernando T. ; Kapralov, Alexandr A. ; Yanamala, Naveena ; Feng, Weihong ; Baygan, Arjang ; ... More +
Andón, Fernando T. ; Kapralov, Alexandr A. ; Yanamala, Naveena ; Feng, Weihong ; Baygan, Arjang ; Chambers, Benedict J. ; Hultenby, Kjell ; Ye, Fei ; Toprak, Muhammet S. ; Brandner, Birgit D. ; Fornara, Andrea ; Klein-Seetharaman, Judith ; Kotchey, Gregg P. ; Star, Alexander ; Shvedova, Anna A. ; Fadeel, Bengt ; Kagan, Valerian E. Less -
Description:
Eosinophil peroxidase (EPO) is one of the major oxidant-producing enzymes during inflammatory states in the human lung. The degradation of single-walled carbon nanotubes (SWCNTs) upon incubation with human EPO and H₂O₂ is reported. Biodegradation of SWCNTs is higher in the presence of NaBr, but neither EPO alone nor H₂O₂ alone caused the degradation of nanotubes. Molecular modeling reveals two binding sites for SWCNTs on EPO, one located at the proximal side (same side as the catalytic site) and the other on the distal side of EPO. The oxidized groups on SWCNTs in both cases are stabilized by electrostatic interactions with positively charged residues. Biodegradation of SWCNTs can also be executed in an ex vivo culture system using primary murine eosinophils stimulated to undergo degranulation. Biodegradation is proven by a range of methods including transmission electron microscopy, UV-visible-NIR spectroscopy, Raman spectroscopy, and confocal Raman imaging. Thus, human EPO (in vitro) and ex vivo activated eosinophils mediate biodegradation of SWCNTs: an observation that is relevant to pulmonary responses to these materials.
Subjects:
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Animals Article Biodegradation, Environmental Eosinophil Peroxidase Eosinophils Humans Mice Nanotubes, Carbon
Source:
Small. 2013; 9(16):2721-2720
Pubmed ID:
23447468
Pubmed Central ID:
PMC4039041
Document Type:
Journal Article
Funding:
R01 HL070755/HL/NHLBI NIH HHSUnited States/ ; R01 OH008282/OH/NIOSH CDC HHSUnited States/ ; U19 AI068021/AI/NIAID NIH HHSUnited States/ ; HL094488/HL/NHLBI NIH HHSUnited States/ ; R01 HL094488/HL/NHLBI NIH HHSUnited States/ ; ... More +
R01 HL070755/HL/NHLBI NIH HHSUnited States/ ; R01 OH008282/OH/NIOSH CDC HHSUnited States/ ; U19 AI068021/AI/NIAID NIH HHSUnited States/ ; HL094488/HL/NHLBI NIH HHSUnited States/ ; R01 HL094488/HL/NHLBI NIH HHSUnited States/ ; R01ES019304/ES/NIEHS NIH HHSUnited States/ ; OH008282/OH/NIOSH CDC HHSUnited States/ ; 3927ZJQP/PHS HHSUnited States/ ; HL70755/HL/NHLBI NIH HHSUnited States/ ; R01 ES019304/ES/NIEHS NIH HHSUnited States/ ; 3927ZHF/PHS HHSUnited States/ ; U19AI068021/AI/NIAID NIH HHSUnited States/ Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:f933ac200157bc3044991a5c245184780e3edaa2e40e483a61116e01c19088f2
Download URL:
https://stacks.cdc.gov/view/cdc/29975/cdc_29975_DS1.pdf
File Type:

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