Biodegradation of Single-Walled Carbon Nanotubes by Eosinophil Peroxidase

Andón, Fernando T.; Kapralov, Alexandr A.; Yanamala, Naveena; Feng, Weihong; Baygan, Arjang; Chambers, Benedict J.; Hultenby, Kjell; Ye, Fei; Toprak, Muhammet S.; Brandner, Birgit D.; Fornara, Andrea; Klein-Seetharaman, Judith; Kotchey, Gregg P.; Star, Alexander; Shvedova, Anna A.; Fadeel, Bengt; Kagan, Valerian E.

doi:10.1002/smll.201202508

Back to Previous Page

- Add this to...
- Twitter
- Facebook
- Google Bookmarks
- LinkedIn

Biodegradation of Single-Walled Carbon Nanotubes by Eosinophil Peroxidase

Published Date:

Feb 27 2013

Publisher's site:

http://dx.doi.org/10.1002/smll.201202508 New Site Icon

Source:

Small. 2013; 9(16):2721-2720.

[PDF-2.41 MB]

Details:

Personal Authors:

Andón, Fernando T. ; Kapralov, Alexandr A. ; Yanamala, Naveena ; Feng, Weihong ; Baygan, Arjang ; ... More ▼

Keywords:

[+]

Pubmed ID:

23447468

Pubmed Central ID:

PMC4039041

Description:

Eosinophil peroxidase (EPO) is one of the major oxidant-producing enzymes during inflammatory states in the human lung. The degradation of single-walled carbon nanotubes (SWCNTs) upon incubation with human EPO and H₂O₂ is reported. Biodegradation of SWCNTs is higher in the presence of NaBr, but neither EPO alone nor H₂O₂ alone caused the degradation of nanotubes. Molecular modeling reveals two binding sites for SWCNTs on EPO, one located at the proximal side (same side as the catalytic site) and the other on the distal side of EPO. The oxidized groups on SWCNTs in both cases are stabilized by electrostatic interactions with positively charged residues. Biodegradation of SWCNTs can also be executed in an ex vivo culture system using primary murine eosinophils stimulated to undergo degranulation. Biodegradation is proven by a range of methods including transmission electron microscopy, UV-visible-NIR spectroscopy, Raman spectroscopy, and confocal Raman imaging. Thus, human EPO (in vitro) and ex vivo activated eosinophils mediate biodegradation of SWCNTs: an observation that is relevant to pulmonary responses to these materials.

Document Type:

Journal Article

Collection(s):

CDC Public Access

Funding:

3927ZHF/PHS HHS/United States
3927ZJQP/PHS HHS/United States
HL094488/HL/NHLBI NIH HHS/United States
HL70755/HL/NHLBI NIH HHS/United States
OH008282/OH/NIOSH CDC HHS/United States
R01 ES019304/ES/NIEHS NIH HHS/United States
R01 HL070755/HL/NHLBI NIH HHS/United States
R01 HL094488/HL/NHLBI NIH HHS/United States
R01ES019304/ES/NIEHS NIH HHS/United States
U19 AI068021/AI/NIAID NIH HHS/United States
U19AI068021/AI/NIAID NIH HHS/United States

Supporting Files:

	nihms583806f4.gif	image/gif
	nihms583806f4.jpg	image/jpeg
	nihms583806f5.gif	image/gif
	nihms583806f5.jpg	image/jpeg
	nihms583806f6.gif	image/gif
	nihms583806f6.jpg	image/jpeg
	NIHMS583806-supplement-Supporting_Information.pdf	application/pdf
	nihms583806.nxml	application/octet-stream
	nihms583806f1.gif	image/gif
	nihms583806f1.jpg	image/jpeg
	nihms583806f2.gif	image/gif
	nihms583806f2.jpg	image/jpeg
	nihms583806f3.gif	image/gif
	nihms583806f3.jpg	image/jpeg

No Related Documents.

You May Also Like:

Enzymatic Oxidative Biodegradation of Nanoparticles: Mechanisms, Significance and Applications

Personal Author:

Vlasova, Irina I.; Kapralov, Alexandr A.; Michael, Zachary P.; Burkert, Seth C.; Shurin, Michael R.; Star, Alexander; Shvedova, Anna A.; Kagan, Valerian E.;

Published:

Jan 06 2016

Source:

Toxicol Appl Pharmacol. 299:58-69.

Description:

Biopersistence of carbon nanotubes, graphene oxide (GO) and several other types of carbonaceous nanomaterials is an essential determinant of their health effects. Successful biodegradation is one of the major factors defining the life span and biolog...

File Type:

[PDF - 2.36 MB]
Peroxidase-mediated Biodegradation of Carbon Nanotubes in vitro and in vivo

Personal Author:

Kotchey, Gregg P.; Zhao, Yong; Kagan, Valerian E.; Star, Alexander;

Published:

Jul 12 2013

Source:

Adv Drug Deliv Rev. 2013; 65(15):1921-1932.

Description:

As a result of their unique electronic, optical, and mechanical properties, carbon nanotubes (CNTs) have been implemented in therapeutic and imaging applications. In an idealized situation, CNTs would be disposed of after they transport their therano...

File Type:

[PDF - 1.12 MB]
Impaired Clearance and Enhanced Pulmonary Inflammatory/Fibrotic Response to Carbon Nanotubes in Myeloperoxidase-Deficient Mice

Personal Author:

Shvedova, Anna A.; Kapralov, Alexandr A.; Feng, Wei Hong; Kisin, Elena R.; Murray, Ashley R.; Mercer, Robert R.; St. Croix, Claudette M.; Lang, Megan A.; Watkins, Simon C.; Konduru, Nagarjun V.; Allen, Brett L.; Conroy, Jennifer; Kotchey, Gregg P.; Mohamed, Bashir M.; Meade, Aidan D.; Volkov, Yuri; Star, Alexander; Fadeel, Bengt; Kagan, Valerian E.;

Published:

Mar 30 2012

Source:

PLoS One. 2012; 7(3).

Description:

Advancement of biomedical applications of carbonaceous nanomaterials is hampered by their biopersistence and pro-inflammatory action in vivo. Here, we used myeloperoxidase knockout B6.129X1-MPO (MPO k/o) mice and showed that oxidation and clearance o...

File Type:

[PDF - 528.83 KB]
Lung Macrophages “Digest” Carbon Nanotubes Using a Superoxide/Peroxynitrite Oxidative Pathway

Personal Author:

Kagan, Valerian E.; Kapralov, Alexandr A.; St. Croix, Claudette M.; Watkins, Simon C.; Kisin, Elena R.; Kotchey, Gregg P.; Balasubramanian, Krishnakumar; Vlasova, Irina I.; Yu, Jaesok; Kim, Kang; Seo, Wanji; Mallampalli, Rama K.; Star, Alexander; Shvedova, Anna A.;

Published:

Jun 04 2014

Source:

ACS Nano. 2014; 8(6):5610-5621.

Description:

In contrast to short-lived neutrophils, macrophages display persistent presence in the lung of animals after pulmonary exposure to carbon nanotubes. While effective in the clearance of bacterial pathogens and injured host cells, the ability of macrop...

File Type:

[PDF - 6.34 MB]
A Natural Vanishing Act: The Enzyme-Catalyzed Degradation of Carbon Nanomaterials

Personal Author:

Kotchey, Gregg P.; Hasan, Saad A.; Kapralov, Alexander A.; Ha, Seung Han; Kim, Kang; Shvedova, Anna A.; Kagan, Valerian E.; Star, Alexander;

Published:

Jul 23 2012

Source:

Acc Chem Res. 45(10):1770-1781.

Description:

Over the past three decades, revolutionary research in nanotechnology by the scientific, medical, and engineering communities has yielded a treasure trove of discoveries with diverse applications that promise to benefit humanity. With their unique el...

File Type:

[PDF - 5.74 MB]