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Biodegradation of Single-Walled Carbon Nanotubes by Eosinophil Peroxidase
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Published Date:
Feb 27 2013
Publisher's site:Source:Small. 2013; 9(16):2721-2720.
Details:
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Alternative Title:Small
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Description:Eosinophil peroxidase (EPO) is one of the major oxidant-producing enzymes during inflammatory states in the human lung. The degradation of single-walled carbon nanotubes (SWCNTs) upon incubation with human EPO and Hâ‚‚Oâ‚‚ is reported. Biodegradation of SWCNTs is higher in the presence of NaBr, but neither EPO alone nor Hâ‚‚Oâ‚‚ alone caused the degradation of nanotubes. Molecular modeling reveals two binding sites for SWCNTs on EPO, one located at the proximal side (same side as the catalytic site) and the other on the distal side of EPO. The oxidized groups on SWCNTs in both cases are stabilized by electrostatic interactions with positively charged residues. Biodegradation of SWCNTs can also be executed in an ex vivo culture system using primary murine eosinophils stimulated to undergo degranulation. Biodegradation is proven by a range of methods including transmission electron microscopy, UV-visible-NIR spectroscopy, Raman spectroscopy, and confocal Raman imaging. Thus, human EPO (in vitro) and ex vivo activated eosinophils mediate biodegradation of SWCNTs: an observation that is relevant to pulmonary responses to these materials.
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Subject:
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Pubmed ID:23447468
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Pubmed Central ID:PMC4039041
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