Details:

Alternative Title:
Small
Publisher's site:
http://dx.doi.org/10.1002/smll.201202508
Personal Author:
Andón, Fernando T. ; Kapralov, Alexandr A. ; Yanamala, Naveena ; Feng, Weihong ; Baygan, Arjang ; ... More +
Andón, Fernando T. ; Kapralov, Alexandr A. ; Yanamala, Naveena ; Feng, Weihong ; Baygan, Arjang ; Chambers, Benedict J. ; Hultenby, Kjell ; Ye, Fei ; Toprak, Muhammet S. ; Brandner, Birgit D. ; Fornara, Andrea ; Klein-Seetharaman, Judith ; Kotchey, Gregg P. ; Star, Alexander ; Shvedova, Anna A. ; Fadeel, Bengt ; Kagan, Valerian E. ; Less -
Description:
Eosinophil peroxidase (EPO) is one of the major oxidant-producing enzymes during inflammatory states in the human lung. The degradation of single-walled carbon nanotubes (SWCNTs) upon incubation with human EPO and H₂O₂ is reported. Biodegradation of SWCNTs is higher in the presence of NaBr, but neither EPO alone nor H₂O₂ alone caused the degradation of nanotubes. Molecular modeling reveals two binding sites for SWCNTs on EPO, one located at the proximal side (same side as the catalytic site) and the other on the distal side of EPO. The oxidized groups on SWCNTs in both cases are stabilized by electrostatic interactions with positively charged residues. Biodegradation of SWCNTs can also be executed in an ex vivo culture system using primary murine eosinophils stimulated to undergo degranulation. Biodegradation is proven by a range of methods including transmission electron microscopy, UV-visible-NIR spectroscopy, Raman spectroscopy, and confocal Raman imaging. Thus, human EPO (in vitro) and ex vivo activated eosinophils mediate biodegradation of SWCNTs: an observation that is relevant to pulmonary responses to these materials.
Subject:
[+]
Animals
Article
Biodegradation, Environmental
Eosinophil Peroxidase
Eosinophils
Humans
Mice
Nanotubes, Carbon

Source:
Small. 2013 ; 9(16):2721-2720.;
Pubmed ID:
23447468
Pubmed Central ID:
PMC4039041
Document Type:
Journal Article ;
Funding:
3927ZHF/PHS HHS/United States ; 3927ZJQP/PHS HHS/United States ; HL094488/HL/NHLBI NIH HHS/United States ; HL70755/HL/NHLBI NIH HHS/United States ; OH008282/OH/NIOSH CDC HHS/United States ; ... More +
3927ZHF/PHS HHS/United States ; 3927ZJQP/PHS HHS/United States ; HL094488/HL/NHLBI NIH HHS/United States ; HL70755/HL/NHLBI NIH HHS/United States ; OH008282/OH/NIOSH CDC HHS/United States ; R01 ES019304/ES/NIEHS NIH HHS/United States ; R01 HL070755/HL/NHLBI NIH HHS/United States ; R01 HL094488/HL/NHLBI NIH HHS/United States ; R01ES019304/ES/NIEHS NIH HHS/United States ; U19 AI068021/AI/NIAID NIH HHS/United States ; U19AI068021/AI/NIAID NIH HHS/United States ; Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:f933ac200157bc3044991a5c245184780e3edaa2e40e483a61116e01c19088f2
File Type:

Supporting Files

• 	nihms583806f4.gif	gif
  	nihms583806f4.jpg	jpeg
  	nihms583806f5.gif	gif
  	nihms583806f5.jpg	jpeg
  	nihms583806f6.gif	gif
  	nihms583806f6.jpg	jpeg
  	NIHMS583806-supplement-Supporting_Information.pdf	pdf
  	nihms583806.nxml	bin
  	nihms583806f1.gif	gif
  	nihms583806f1.jpg	jpeg
  	nihms583806f2.gif	gif
  	nihms583806f2.jpg	jpeg
  	nihms583806f3.gif	gif
  	nihms583806f3.jpg	jpeg

More +