Lomaiviticin Biosynthesis Employs a New Strategy for Starter Unit Generation

Details

• Alternative Title:
  Org Lett
• Personal Author:
  Waldman, Abraham J. ; Balskus*, Emily P.
• Description:
  Lomaiviticin biosynthesis is thought to utilize a propionyl starter unit for a type II polyketide synthase (PKS). Discovery of the lomaiviticin (lom) biosynthetic gene cluster suggested an unusual method for starter unit generation involving a bifunctional acyltransferase/decarboxylase (AT/DC) thus far observed only in type I PKS pathways. In vitro biochemical characterization of AT/DC Lom62 confirmed its ability to generate a propionyl-acyl carrier protein (ACP), revealing a new role for this enzymatic activity within natural product biosynthesis.
• Subjects:
  Acyl Carrier Protein Acyltransferases Amino Acid Sequence Fluorenes Molecular Structure Multigene Family Polyketide Synthases Streptomyces
• Source:
  Org Lett. 16(2):640-643
• Pubmed ID:
  24383813
• Pubmed Central ID:
  PMC3965344
• Document Type:
  Journal Article
• Funding:
  DP2 GM105434/GM/NIGMS NIH HHSUnited States/ ; T32 GM095450/GM/NIGMS NIH HHSUnited States/ ; GM095450/GM/NIGMS NIH HHSUnited States/ ; DP2 GM105434/DP/NCCDPHP CDC HHSUnited States/
• Volume:
  16
• Issue:
  2
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:f2505bccc95bc1830afe40aaf3563067e72e86786eb8f2a664bb00e7d19ee2d6
• Download URL:
  https://stacks.cdc.gov/view/cdc/29892/cdc_29892_DS1.pdf
• File Type:
  [PDF - 642.01 KB ]