Lomaiviticin Biosynthesis Employs a New Strategy for Starter Unit Generation

Waldman, Abraham J.; Balskus*, Emily P.

doi:10.1021/ol403714g

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Lomaiviticin Biosynthesis Employs a New Strategy for Starter Unit Generation

Published Date:

Jan 02 2014

Publisher's site:

http://dx.doi.org/10.1021/ol403714g New Site Icon

Source:

Org Lett. 16(2):640-643.

[PDF-642.01 KB]

Details:

Personal Authors:

Waldman, Abraham J. ; Balskus*, Emily P. ;

Keywords:

[+]

Pubmed ID:

24383813

Pubmed Central ID:

PMC3965344

Description:

Lomaiviticin biosynthesis is thought to utilize a propionyl starter unit for a type II polyketide synthase (PKS). Discovery of the lomaiviticin (lom) biosynthetic gene cluster suggested an unusual method for starter unit generation involving a bifunctional acyltransferase/decarboxylase (AT/DC) thus far observed only in type I PKS pathways. In vitro biochemical characterization of AT/DC Lom62 confirmed its ability to generate a propionyl-acyl carrier protein (ACP), revealing a new role for this enzymatic activity within natural product biosynthesis.

Document Type:

Journal Article

Collection(s):

CDC Public Access

Funding:

DP2 GM105434/DP/NCCDPHP CDC HHS/United States
DP2 GM105434/GM/NIGMS NIH HHS/United States
GM095450/GM/NIGMS NIH HHS/United States
T32 GM095450/GM/NIGMS NIH HHS/United States

Supporting Files:

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