Calibration-free quantification of absolute oxygen saturation based on the dynamics of photoacoustic signals

Details

• Alternative Title:
  Opt Lett
• Personal Author:
  Xia, Jun ; Danielli, Amos ; Liu, Yan ; Wang, Lidai ; Maslov, Konstantin ; Wang, Lihong V.
• Description:
  Photoacoustic tomography (PAT) is a hybrid imaging technique that has broad preclinical and clinical applications. Based on the photoacoustic effect, PAT directly measures specific optical absorption, which is the product of the tissue-intrinsic optical absorption coefficient and the local optical fluence. Therefore, quantitative PAT, such as absolute oxygen saturation (sO₂) quantification, requires knowledge of the local optical fluence, which can only be estimated through invasive measurements or sophisticated modeling of light transportation. In this Letter, we circumvent this requirement by taking advantage of the dynamics in sO₂. The new method works when the sO₂ transition can be simultaneously monitored with multiple wavelengths. For each wavelength, the ratio of photoacoustic amplitudes measured at different sO₂ states is utilized. Using the ratio cancels the contribution from optical fluence and allows calibration-free quantification of absolute sO₂. The new method was validated through both phantom and in vivo experiments.
• Subjects:
  Animals Article Calibration Cerebral Cortex Mice Oxygen Phantoms, Imaging Photoacoustic Techniques Tomography, X-Ray Computed
• Source:
  Opt Lett. 2010; 38(15):2800-2803.
• Pubmed ID:
  23903146
• Pubmed Central ID:
  PMC3884570
• Document Type:
  Journal Article
• Funding:
  R01 CA134539/CA/NCI NIH HHS/United States ; R01 CA157277/CA/NCI NIH HHS/United States ; U54 CA136398/CA/NCI NIH HHS/United States ; R01 CA159959/CA/NCI NIH HHS/United States ; DP1 EB016986/EB/NIBIB NIH HHS/United States ; R01 EB008085/EB/NIBIB NIH HHS/United States ; DP1 EB016986/DP/NCCDPHP CDC HHS/United States
• Volume:
  38
• Issue:
  15
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:7fb8e9849aab978327b7dd53caba6cedf904e079c3e9ce97da22967c83fb4de0
• Download URL:
  https://stacks.cdc.gov/view/cdc/29822/cdc_29822_DS1.pdf
• File Type:
  [PDF - 624.33 KB ]