Random-access optical-resolution photoacoustic microscopy using a digital micromirror device

Details

• Alternative Title:
  Opt Lett
• Personal Author:
  Liang, Jinyang ; Zhou, Yong ; Winkler, Amy W. ; Wang, Lidai ; Maslov, Konstantin I. ; Li, Chiye ; Wang, Lihong V.
• Description:
  We developed random-access optical-resolution photoacoustic microscopy using a digital micromirror device. This system can rapidly scan arbitrarily shaped regions of interest within a 40 μm×40 μm imaging area with a lateral resolution of 3.6 μm. To identify a region of interest, a global structural image is first acquired, then the selected region is scanned. The random-access ability was demonstrated by imaging two static samples, a carbon fiber cross and a monolayer of red blood cells, with an acquisition rate up to 4 kHz. The system was then used to monitor blood flow in vivo in real time within user-selected capillaries in a mouse ear. By imaging only the capillary of interest, the frame rate was increased by up to 9.2 times.
• Subjects:
  Animals Article Ear Mice Microscopy Optical Devices Photoacoustic Techniques
• Source:
  Opt Lett. 2013; 38(15):2683-2686.
• Pubmed ID:
  23903111
• Pubmed Central ID:
  PMC3784350
• Document Type:
  Journal Article
• Funding:
  T32 EB014855/EB/NIBIB NIH HHS/United States ; R01 CA134539/CA/NCI NIH HHS/United States ; R01 CA157277/CA/NCI NIH HHS/United States ; U54 CA136398/CA/NCI NIH HHS/United States ; R01 CA159959/CA/NCI NIH HHS/United States ; R01 EB010049/EB/NIBIB NIH HHS/United States ; DP1 EB016986/EB/NIBIB NIH HHS/United States ; R01 EB008085/EB/NIBIB NIH HHS/United States ; DP1 EB016986/DP/NCCDPHP CDC HHS/United States
• Volume:
  38
• Issue:
  15
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:f5f785ff64e74ac11b514c92865511e8daeb539fa47d819475b30b384dda4f8f
• Download URL:
  https://stacks.cdc.gov/view/cdc/29746/cdc_29746_DS1.pdf
• File Type:
  [PDF - 866.30 KB ]