Random-access optical-resolution photoacoustic microscopy using a digital micromirror device

Liang, Jinyang; Zhou, Yong; Winkler, Amy W.; Wang, Lidai; Maslov, Konstantin I.; Li, Chiye; Wang, Lihong V.

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Random-access optical-resolution photoacoustic microscopy using a digital micromirror device

Published Date:

Aug 01 2013

Source:

Opt Lett. 2013; 38(15):2683-2686.

Language:

English

[PDF-866.30 KB]

Details:

Personal Authors:

Liang, Jinyang ; Zhou, Yong ; Winkler, Amy W. ; Wang, Lidai ; Maslov, Konstantin I. ; ... More ▼

Keywords:

[+]

Pubmed ID:

23903111

Pubmed Central ID:

PMC3784350

Description:

We developed random-access optical-resolution photoacoustic microscopy using a digital micromirror device. This system can rapidly scan arbitrarily shaped regions of interest within a 40 μm×40 μm imaging area with a lateral resolution of 3.6 μm. To identify a region of interest, a global structural image is first acquired, then the selected region is scanned. The random-access ability was demonstrated by imaging two static samples, a carbon fiber cross and a monolayer of red blood cells, with an acquisition rate up to 4 kHz. The system was then used to monitor blood flow in vivo in real time within user-selected capillaries in a mouse ear. By imaging only the capillary of interest, the frame rate was increased by up to 9.2 times.

Document Type:

Journal Article

Collection(s):

CDC Public Access

Funding:

T32 EB014855/EB/NIBIB NIH HHS/United States
R01 CA134539/CA/NCI NIH HHS/United States
R01 CA157277/CA/NCI NIH HHS/United States
U54 CA136398/CA/NCI NIH HHS/United States
R01 CA159959/CA/NCI NIH HHS/United States
R01 EB010049/EB/NIBIB NIH HHS/United States
DP1 EB016986/EB/NIBIB NIH HHS/United States
R01 EB008085/EB/NIBIB NIH HHS/United States
DP1 EB016986/DP/NCCDPHP CDC HHS/United States

Supporting Files:

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