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Comparison of whole genome sequences from human and non-human Escherichia coli O26 strains
  • Published Date:
    Mar 11 2015
  • Source:
    Front Cell Infect Microbiol. 2015; 5.
Filetype[PDF - 1.40 MB]


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  • Description:
    Shiga toxin-producing | (STEC) O26 is the second leading | serogroup responsible for human illness outbreaks behind | O157:H7. Recent outbreaks have been linked to emerging pathogenic O26:H11 strains harboring | only. Cattle have been recognized as an important reservoir of O26 strains harboring |; however the reservoir of these emerging | strains is unknown. The objective of this study was to identify nucleotide polymorphisms in human and cattle-derived strains in order to compare differences in polymorphism derived genotypes and virulence gene profiles between the two host species. Whole genome sequencing was performed on 182 epidemiologically unrelated O26 strains, including 109 human-derived strains and 73 non-human-derived strains. A panel of 289 O26 strains (241 STEC and 48 non-STEC) was subsequently genotyped using a set of 283 polymorphisms identified by whole genome sequencing, resulting in 64 unique genotypes. Phylogenetic analyses identified seven clusters within the O26 strains. The seven clusters did not distinguish between isolates originating from humans or cattle; however, clusters did correspond with particular virulence gene profiles. Human and non-human-derived strains harboring | clustered separately from strains harboring |, strains harboring |, and non-STEC strains. Strains harboring | were more closely related to non-STEC strains and strains harboring | than to strains harboring |. The finding of human and cattle-derived strains with the same polymorphism derived genotypes and similar virulence gene profiles, provides evidence that similar strains are found in cattle and humans and transmission between the two species may occur.