A Carbonate-Forming Baeyer-Villiger Monooxygenase

Details

• Alternative Title:
  Nat Chem Biol
• Personal Author:
  Hu, Youcai ; Dietrich, David ; Xu, Wei ; Patel, Ashay ; Thuss, Justin A. J. ; Wang, Jingjing ; Yin, Wen-Bing ; Qiao, Kangjian ; Houk, Kendall N. ; Vederas, John C. ; Tang, Yi
• Description:
  Despite the remarkable versatility displayed by flavin-dependent monooxygenases (FMOs) in natural product biosynthesis, one notably missing activity is the oxidative generation of carbonate functional groups. We describe a multifunctional Baeyer-Villiger monooxygenase, CcsB, which catalyzes the formation of an in-line carbonate in the macrocyclic portion of cytochalasin E. This study expands the repertoire of activities of FMOs and provides a possible synthetic strategy for transformation of ketones into carbonates.
• Subjects:
  Amino Acid Sequence Aspergillus Carbonates Catalysis Cytochalasins Fungal Proteins Gene Expression Regulation, Fungal Gene Knockout Techniques Genetic Complementation Test Ketones Mixed Function Oxygenases Oxidation-Reduction

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• Source:
  Nat Chem Biol. 10(7):552-554
• Pubmed ID:
  24838010
• Pubmed Central ID:
  PMC4062580
• Document Type:
  Journal Article
• Funding:
  DP1 GM106413/GM/NIGMS NIH HHSUnited States/ ; T32GM008496/GM/NIGMS NIH HHSUnited States/ ; 1R01GM085128/GM/NIGMS NIH HHSUnited States/ ; T32 GM008496/GM/NIGMS NIH HHSUnited States/ ; 1DP1GM106413/DP/NCCDPHP CDC HHSUnited States/ ; R01 GM085128/GM/NIGMS NIH HHSUnited States/
• Volume:
  10
• Issue:
  7
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha-512:b51006f772f2a01e312d01ba613a756680b52ea13c4959594582d425924e8e57fbaa6245af09e62bc9a87051cb5fe3051fc5c23211b53ec5937f228b809988d7
• Download URL:
  https://stacks.cdc.gov/view/cdc/27888/cdc_27888_DS1.pdf
• File Type:
  [PDF - 664.66 KB ]