Details:

Alternative Title:
Nat Chem Biol
Personal Author:
Hu, Youcai ; Dietrich, David ; Xu, Wei ; Patel, Ashay ; Thuss, Justin A. J. ; ... More +
Hu, Youcai ; Dietrich, David ; Xu, Wei ; Patel, Ashay ; Thuss, Justin A. J. ; Wang, Jingjing ; Yin, Wen-Bing ; Qiao, Kangjian ; Houk, Kendall N. ; Vederas, John C. ; Tang, Yi ; Less -
Description:
Despite the remarkable versatility displayed by flavin-dependent monooxygenases (FMOs) in natural product biosynthesis, one notably missing activity is the oxidative generation of carbonate functional groups. We describe a multifunctional Baeyer-Villiger monooxygenase, CcsB, which catalyzes the formation of an in-line carbonate in the macrocyclic portion of cytochalasin E. This study expands the repertoire of activities of FMOs and provides a possible synthetic strategy for transformation of ketones into carbonates.
Subject:
[+]
Amino Acid Sequence
Article
Aspergillus
Carbonates
Catalysis
Cytochalasins
Fungal Proteins
Gene Expression Regulation, Fungal
Gene Knockout Techniques
Genetic Complementation Test
Ketones
Mixed Function Oxygenases
Oxidation-Reduction

Source:
Nat Chem Biol. 10(7):552-554. ;
Pubmed ID:
24838010
Pubmed Central ID:
PMC4062580
Document Type:
Journal Article ;
Funding:
1DP1GM106413/DP/NCCDPHP CDC HHS/United States ; 1R01GM085128/GM/NIGMS NIH HHS/United States ; DP1 GM106413/GM/NIGMS NIH HHS/United States ; R01 GM085128/GM/NIGMS NIH HHS/United States ; T32GM008496/GM/NIGMS NIH HHS/United States ;
1DP1GM106413/DP/NCCDPHP CDC HHS/United States ; 1R01GM085128/GM/NIGMS NIH HHS/United States ; DP1 GM106413/GM/NIGMS NIH HHS/United States ; R01 GM085128/GM/NIGMS NIH HHS/United States ; T32GM008496/GM/NIGMS NIH HHS/United States ; Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:ecc5c1a8442d346789e3a4610036e4e220c3138f0e0c81d8d5476f64efc6be7b
File Type:

Supporting Files

• 	NIHMS585726-supplement-1.pdf	pdf
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