Comparison of Two Interferon-Gamma Release Assays For Pediatric Tuberculosis Infection.

Details

• Alternative Title:
  Journal of the Pediatric Infectious Diseases Society, 2025, v. 14, no. 1: Comparison of Two Interferon-Gamma Release Assays For Pediatric Tuberculosis Infection.
• Journal Article:
  Journal of the Pediatric Infectious Diseases Society
• Personal Author:
  Gaensbauer, James T. ; Reves, Randall R. ; Katz, Dolly ; Ahmed, Amina ; Venkatappa, Thara
• Corporate Authors:
  for the Tuberculosis Epidemiologic Studies Consortium
• Description:
  Identifying tuberculosis infection (TBI) using interferon-gamma release assays (IGRAs) is a primary component of clinical and public health efforts to prevent pediatric tuberculosis (TB). Pediatric data comparing the 2 IGRAs in the United States are very limited. We compared the performance of the 2 IGRAs among a large pediatric cohort tested for TBI and assessed whether discordance might be due to quantitative results close to test cutoff values.
  
  Children aged 0-15 years with both T-SPOT.TB (T-SPOT) and QuantiFERON-TB Gold In-Tube (QFT-GIT) tests were identified from a US multicenter study enrolling people at elevated risk of TBI or progression to TB disease. Results were compared using McNemar's Chi-square tests with stratification by age category and testing reason. Percent agreement and kappa statistics were also calculated. We characterized quantitative test results among children with discordant QFT-GIT-positive/T-SPOT-negative results.
  
  Among 3793 children, a higher number had positive QFT-GIT than T-SPOT (10.1% vs 7.4%, P < .001). This difference was noted for all age categories except <2 years, and for children with close-contact and non-close contact test indications. Among discordant QFT-GIT-positive/T-SPOT-negative children, lowering the positive threshold for T-SPOT to include borderline spot counts (5-7) did not eliminate the discordance, nor were QFT-GIT antigen-minus-nil results concentrated in the range just above the standard cutoff of 0.35 IU/mL.
  
  In a large pediatric cohort tested for TBI, QFT-GIT had a higher proportion of positive results than T-SPOT, and discordance was not related to quantitative results close to the established diagnostic cutoffs.
• Content Notes:
  Author manuscript
• Subjects:
  • Adolescent
  • Child
  • Child, Preschool
  • Female
  • Humans
  • Infant
  • Infant, Newborn
  • Male
  • Interferon-gamma Release Tests
  • Sensitivity and Specificity
  More +
• Keywords:
  • T-SPOT.
  • TB
  • children
  • tuberculosis
  • pediatric
  • tuberculosis infection
  • QuantiFERON-TB Gold In-Tube
• Source:
  Journal of the Pediatric Infectious Diseases Society, 2025, v. 14, no. 1
• DOI:
  https://doi.org/10.1093/jpids/piae135
• ISSN:
  2048-7193 ; 2048-7207
• Pubmed ID:
  39749968
• Pubmed Central ID:
  PMC11851244
• Document Type:
  Text
• Funding:
  CC999999/ImCDC/Intramural CDC HHSUnited States/ ; CC/CDC HHSUnited States/
• Genre:
  Journal Article
• Place as Subject:
  United States
• Pages in Document:
  piae135(16 pdf pages)
• Volume:
  14
• Issue:
  1
• Download URL:
  https://stacks.cdc.gov/view/cdc/261809/cdc_261809_DS1.pdf
• File Type:
  [PDF - 468.63 KB]
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha-512:3220c8d92b73ea7ce9657573d901c0b588206049b97f612ceb6ed57980893ef9469b30d91d28c82e4c2acb4ae9f017f79e2b2edab1338cb73d1f71cb3926fa84