Rapid and Sensitive Detection of Wood Smoke Exposure Biomarkers Using Europium Fluorescent Nanoparticle Label/lateral Flow Immunoassay.

Details

• Alternative Title:
  Talanta, 2025, v. 291: Rapid and Sensitive Detection of Wood Smoke Exposure Biomarkers Using Europium Fluorescent Nanoparticle Label/lateral Flow Immunoassay.
• Journal Article:
  Talanta
• Personal Author:
  Fu, Yonghao ; Song, Yang ; Yang, Zhansen ; Ruan, Xiaofan ; Lin, Yuehe ; Du, Dan
• Description:
  Exposure to wood smoke is associated with various adverse health problems. Biomonitoring of smoke exposure-associated biomarkers provides accurate measurements of personally absorbed doses. As a specific metabolite of benzene, the quantitative measurement of S-phenylmercapturic acid (S-PMA) plays a vital role in evaluating human exposure to wood smoke. In this study, we developed an efficient lateral flow immunoassay (LFIA) approach for accurately and rapidly measuring S-PMA levels. Europium chelate nanoparticles (EuNPs) conjugated with purified polyclonal sheep anti-S-PMA antibodies were employed as the fluorescent detection probe. This work is based on a competitive immunoassay, where the target S-PMA competes with the immobilized antigen on the test lines for the limited antigen-binding sites on EuNP-conjugated antibodies. Due to this competition, the fluorescent intensity of the EuNPs is inversely proportional to the concentration of the target S-PMA in the sample, enabling quantitative measurement. Owing to the large Stokes shift, superior fluorescent brightness, and saturation of the EuNPs, S-PMA levels can be measured with a limit of detection of 0.32 ng/mL, a detectable range of 0.10-30 ng/mL, and a linear detection range of 0.25-30 ng/mL under optimized conditions. Stability testing revealed that the LFIA strips can be stored at room temperature for up to one year while maintaining excellent detection performance for S-PMA. These results demonstrate that the EuNP-based LFIA is a promising tool for accurate preclinical and point-of-care evaluation of wood smoke exposure. A major advantage of this approach is its ability to accurately analyze smoke biomarkers at anticipated low concentrations. The sensor system allows low-cost, rapid, and on-site data collection and quantification of wood smoke exposure.
• Content Notes:
  Author manuscript
• Subjects:
  • Biomarkers
  • Wood
  • Smoke
  • Europium
  • Immunoassay
  • Fluorescent Dyes
  • Nanoparticles
  • Humans
  • Limit of Detection
  • Animals
• Keywords:
  • Lateral flow immunoassay
  • Europium chelates nanoparticles
  • Wood smoke biomarker
  • S-phenylmercapturic acid
• Source:
  Talanta, 2025, v. 291
• DOI:
  https://doi.org/10.1016/j.talanta.2025.127760
• ISSN:
  0039-9140 ; 1873-3573
• Pubmed ID:
  40024128
• Pubmed Central ID:
  PMC12011643
• Document Type:
  Text
• Funding:
  R01 OH012579/OH/NIOSH CDC HHSUnited States/
• Genre:
  Journal Article
• Pages in Document:
  127760 (23 pdf pages)
• Volume:
  291
• Download URL:
  https://stacks.cdc.gov/view/cdc/261639/cdc_261639_DS1.pdf
• File Type:
  [PDF - 2.32 MB]
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha-512:80f7738b4d64d129f9399330e2e59ad14d9543dfb5195b6914cf00fc8077c835a80efa875ecfe6b5abf15f1f7cda186ae370904647852745ed029c0347ca446d