Airborne Bacteria in Institutional and Commercial Buildings in Korea: Characterization With 16S RRNA Gene Sequencing and Association With Environmental Conditions.

Details

• Alternative Title:
  Aerosol Science and Technology : the Journal of the American Association for Aerosol Research, 2025, v. 58, no. 11: Airborne Bacteria in Institutional and Commercial Buildings in Korea: Characterization With 16S RRNA Gene Sequencing and Association With Environmental Conditions.
• Journal Article:
  Aerosol Science and Technology : the Journal of the American Association for Aerosol Research
• Personal Author:
  Kim, Yea Joon ; Lee, Bong Gu ; Shim, Joo Eun ; Lee, Hyesoo ; Park, Ju-Hyeong ; Yeo, Min-Kyeong
• Description:
  Information on microorganisms in indoor air of various institutional and commercial buildings has significant value in a public health management perspective. However, there is a lack of prior research comparing indoor airborne microbiota across different categories of those buildings. We characterized indoor airborne bacteria in 10 buildings (two for each of five categories: train station, parking garage, mart, public library, and daycare center) during summer and winter. The 16S rRNA gene in the bacterial gDNA extracted from samples was quantified using quantitative real-time polymerase chain reaction and sequenced with the Illumina MiSeq
  
  platform for characterizing community composition. We collected information on temperature, relative humidity, CO concentration, and particulate matter (PM) concentrations by particle size (<1μm, 1-2.5μm, 2.5-10μm) indoors. We performed a multivariate regression analysis to identify factors influencing bacterial quantity and Permutational Multivariate Analysis of Variance (PERMANOVA) to determine factors affecting cluster dissimilarity. We found that bacterial concentration was significantly (-values < 0.05) associated with season and CO concentration. The PERMANOVA analyses showed the significant (-values < 0.05) associations of bacterial cluster dissimilarity with season, building category, and CO. Our study indicated that the season, and CO concentrations may be important factors associated with the indoor airborne bacterial concentration and composition. Building category and usage appeared to significantly influence the bacterial community composition but not the concentration. Our study may provide basic data on bacterial community composition and their concentration that are needed for properly managing microbial exposures in occupants or customers of the studied institutional and commercial buildings.
• Content Notes:
  Author manuscript
• Subjects:
  • Air Pollution, Indoor
  • Genes, rRNA
  • Humidity
  • Particle Size
  • Public Health
  • RNA, Ribosomal, 16S
  • Real-Time Polymerase Chain Reaction
  • Seasons
  • Temperature
  • Multivariate Analysis
  More +
• Keywords:
  • Indoor air
  • Bacteria
  • 16S rRNA
  • Building indoor air
  • DNA sequencing
  • Public-Use Facility
• Source:
  Aerosol Science and Technology : the Journal of the American Association for Aerosol Research, 2025, v. 58, no. 11
• DOI:
  https://doi.org/10.1080/02786826.2024.2387135
• ISSN:
  0278-6826
• Pubmed ID:
  40125263
• Pubmed Central ID:
  PMC11926998
• Document Type:
  Text
• Funding:
  CC999999/ImCDC/Intramural CDC HHSUnited States/
• Genre:
  Journal Article
• Place as Subject:
  Korea
• Pages in Document:
  1281-1292 (23 pdf pages)
• Volume:
  58
• Issue:
  11
• Download URL:
  https://stacks.cdc.gov/view/cdc/261571/cdc_261571_DS1.pdf
• File Type:
  [PDF - 912.54 KB]
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha-512:75541e1209809db86b1d4ea9c3c3c05681815cb206f09445c3e9452de4e3b8cf7f51f7a1ffef0f353a8508a17130cab53be665edbc1340e31c689e67da4b157a