TEMI: Tissue-expansion Mass-spectrometry Imaging.

Details

• Alternative Title:
  Nature Methods, 2025, v. 22, no. 5: TEMI: Tissue-expansion Mass-spectrometry Imaging.
• Journal Article:
  Nature Methods
• Personal Author:
  Zhang, Hua ; Ding, Lang ; Hu, Amy ; Shi, Xudong ; Huang, Penghsuan ; Lu, Haiyan ; Tillberg, Paul W. ; Wang, Meng C. ; Li, Lingjun
• Description:
  The spatial distribution of diverse biomolecules in multicellular organisms is essential for their physiological functions. High-throughput in situ mapping of biomolecules is crucial for both basic and medical research, and requires high scanning speed, spatial resolution, and chemical sensitivity. Here we developed a tissue-expansion method compatible with matrix-assisted laser desorption/ionization mass-spectrometry imaging (TEMI). TEMI reaches single-cell spatial resolution without sacrificing voxel throughput and enables the profiling of hundreds of biomolecules, including lipids, metabolites, peptides (proteins), and N-glycans. Using TEMI, we mapped the spatial distribution of biomolecules across various mammalian tissues and uncovered metabolic heterogeneity in tumors. TEMI can be easily adapted and broadly applied in biological and medical research, to advance spatial multi-omics profiling.
• Content Notes:
  Author manuscript
• Subjects:
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Animals
  • Mice
  • Humans
  • Single-Cell Analysis
• Keywords:
  • Metabolomics
  • Mass spectrometry
  • Molecular imaging
  • Lipidomics
• Source:
  Nature Methods, 2025, v. 22, no. 5
• DOI:
  https://doi.org/10.1038/s41592-025-02664-9
• ISSN:
  1548-7091 ; 1548-7105
• Pubmed ID:
  40263584
• Pubmed Central ID:
  PMC12074994
• Document Type:
  Text
• Funding:
  DP1DK113644/U.S. Department of Health & Human Services ; National Institutes of Health (NIH)/ ; S10 OD025084/OD/NIH HHSUnited States/ ; S10 OD028473/CD/ODCDC CDC HHSUnited States/ ; S10 RR029531/RR/NCRR NIH HHSUnited States/ ; P30 DK020579/DK/NIDDK NIH HHSUnited States/ ; DP1 DK113644/DK/NIDDK NIH HHSUnited States/ ; R01 DK071801/DK/NIDDK NIH HHSUnited States/ ; P01 CA250972/CA/NCI NIH HHSUnited States/ ; S10 OD028473/OD/NIH HHSUnited States/ ; R01 AG052324/AG/NIA NIH HHSUnited States/ ; R01 AG078794/AG/NIA NIH HHSUnited States/ ; R01 AG078794, R01 DK071801, R01 AG052324/U.S. Department of Health & Human Services ; National Institutes of Health (NIH)/
• Genre:
  Journal Article
• Pages in Document:
  1051-1058 (25 pdf pages)
• Volume:
  22
• Issue:
  5
• Download URL:
  https://stacks.cdc.gov/view/cdc/261464/cdc_261464_DS1.pdf
• File Type:
  [PDF - 6.57 MB]
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha-512:3371fd4cf0cc74b70742df5bd0051039af8efe17b64c4007732d642ffbc1f01c83315d7bf680d6bebdac3907f9a96c2290a5be33a93127c67ddbe4b32b3e5d20