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Mapping of Phospholipids by MALDI Imaging (MALDI-MSI): Realities and Expectations.
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  • Description:
    Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has emerged as a novel powerful MS methodology that has the ability to generate both molecular and spatial information within a tissue section. Application of this technology as a new type of biochemical lipid microscopy may lead to new discoveries of the lipid metabolism and biomarkers associated with area-specific alterations or damage under stress/disease conditions such as traumatic brain injury or acute lung injury, among others. However there are limitations in the range of what it can detect as compared with liquid chromatography-MS (LC-MS) of a lipid extract from a tissue section. The goal of the current work was to critically consider remarkable new opportunities along with the limitations and approaches for further improvements of MALDI-MSI. Based on our experimental data and assessments, improvements of the spectral and spatial resolution, sensitivity and specificity towards low abundance species of lipids are proposed. This is followed by a review of the current literature, including methodologies that other laboratories have used to overcome these challenges.

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  • Funding:
    ES020693/ES/NIEHS NIH HHS/United States
    ES021068/ES/NIEHS NIH HHS/United States
    HL094488/HL/NHLBI NIH HHS/United States
    HL70755/HL/NHLBI NIH HHS/United States
    NS076511/NS/NINDS NIH HHS/United States
    OH008282/OH/NIOSH CDC HHS/United States
    P30 CA047904/CA/NCI NIH HHS/United States
    P30 CA047904/CA/NCI NIH HHS/United States
    P50 NS030318/NS/NINDS NIH HHS/United States
    R01 ES020693/ES/NIEHS NIH HHS/United States
    R01 HL070755/HL/NHLBI NIH HHS/United States
    R01 HL094488/HL/NHLBI NIH HHS/United States
    R01 NS061817/NS/NINDS NIH HHS/United States
    R01 NS076511/NS/NINDS NIH HHS/United States
    R21 ES021068/ES/NIEHS NIH HHS/United States
    R37 HL065697/HL/NHLBI NIH HHS/United States
    U19 AI068021/AI/NIAID NIH HHS/United States
    U19AI068021/AI/NIAID NIH HHS/United States
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