Mapping of Phospholipids by MALDI Imaging (MALDI-MSI): Realities and Expectations.

Details

• Alternative Title:
  Chem Phys Lipids
• Personal Author:
  Sparvero, L.J. ; Amoscato, A.A. ; Dixon, C.E. ; Long, J.B. ; Kochanek, P.M. ; Pitt, B.R. ; Bayir, H. ; Kagan, V.E.
• Description:
  Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has emerged as a novel powerful MS methodology that has the ability to generate both molecular and spatial information within a tissue section. Application of this technology as a new type of biochemical lipid microscopy may lead to new discoveries of the lipid metabolism and biomarkers associated with area-specific alterations or damage under stress/disease conditions such as traumatic brain injury or acute lung injury, among others. However there are limitations in the range of what it can detect as compared with liquid chromatography-MS (LC-MS) of a lipid extract from a tissue section. The goal of the current work was to critically consider remarkable new opportunities along with the limitations and approaches for further improvements of MALDI-MSI. Based on our experimental data and assessments, improvements of the spectral and spatial resolution, sensitivity and specificity towards low abundance species of lipids are proposed. This is followed by a review of the current literature, including methodologies that other laboratories have used to overcome these challenges.
• Subjects:
  Brain Dietary Supplements Docosahexaenoic Acids Molecular Imaging Phospholipids Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
• Keywords:
  Acute Lung Injury Docosahexaenoic Acid Lipidomics Lipid Peroxidation MALDI Imaging Traumatic Brain Injury
• Source:
  Chem Phys Lipids. 2012; 165(5):545-562
• Pubmed ID:
  22692104
• Pubmed Central ID:
  PMC3642772
• Document Type:
  Journal Article
• Funding:
  R01 HL070755/HL/NHLBI NIH HHSUnited States/ ; R01 OH008282/OH/NIOSH CDC HHSUnited States/ ; U19 AI068021/AI/NIAID NIH HHSUnited States/ ; ES021068/ES/NIEHS NIH HHSUnited States/ ; R01 NS076511/NS/NINDS NIH HHSUnited States/ ; ES020693/ES/NIEHS NIH HHSUnited States/ ; R01 HL094488/HL/NHLBI NIH HHSUnited States/ ; P50 NS030318/NS/NINDS NIH HHSUnited States/ ; NS076511/NS/NINDS NIH HHSUnited States/ ; U19AI068021/AI/NIAID NIH HHSUnited States/ ; HL70755/HL/NHLBI NIH HHSUnited States/ ; R21 ES021068/ES/NIEHS NIH HHSUnited States/ ; R37 HL065697/HL/NHLBI NIH HHSUnited States/ ; R01 ES020693/ES/NIEHS NIH HHSUnited States/ ; P30 CA047904/CA/NCI NIH HHSUnited States/ ; P01 NS030318/NS/NINDS NIH HHSUnited States/ ; R37 NS061817/NS/NINDS NIH HHSUnited States/ ; HL094488/HL/NHLBI NIH HHSUnited States/ ; OH008282/OH/NIOSH CDC HHSUnited States/ ; R01 NS061817/NS/NINDS NIH HHSUnited States/
• Volume:
  165
• Issue:
  5
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha-512:77f1fbb03722e6ea56e92654a552d063126bf7d3bff87c4f0fb22d20d5662897bddace37c9989f338359b664b7b4ca40ad0c604cea683528e85b49b9fa843201
• Download URL:
  https://stacks.cdc.gov/view/cdc/33549/cdc_33549_DS1.pdf
• File Type:
  [PDF - 4.50 MB ]