Effect of Temperature and Time on RNA Detection by RT-qPCR in Rodent Tissue and Blood Samples Stored in MagMAX™ Lysis/Binding Solution Concentrate: Considerations For RNA Detection in Specimens Stored Under Suboptimal Conditions.

Details

• Alternative Title:
  Journal of Virological Methods, 2025, v. 337: Effect of Temperature and Time on RNA Detection by RT-qPCR in Rodent Tissue and Blood Samples Stored in MagMAX™ Lysis/Binding Solution Concentrate: Considerations For RNA Detection in Specimens Stored Under Suboptimal Conditions.
• Journal Article:
  Journal of Virological Methods
• Personal Author:
  Davies, Katherine A. ; Welch, Stephen R. ; Sorvillo, Teresa E. ; Coleman-McCray, JoAnn D. ; Spiropoulou, Christina F. ; Spengler, Jessica R.
• Description:
  For detection of viral RNA in blood or tissue, samples are often collected into lysis buffers prior to downstream molecular analysis. Immediate sample processing and cold storage are not always possible during large-scale or field studies, or in facilities lacking a stable electrical supply. Additionally, samples may need to be transported significant distances before processing. Here, using peptidylprolyl isomerase A (Ppia), a stably expressed gene in rodent tissues, we investigate the long-term stability and detection of RNA in guinea pig tissues stored for up to 52 weeks and in hamster blood stored for up to 12 weeks in MagMAX Lysis/Binding Solution Concentrate at -80°C, 4°C, 21°C, and 32°C.
• Content Notes:
  Author manuscript
• Subjects:
  • Animals
  • Specimen Handling
  • Temperature
  • RNA, Viral
  • Guinea Pigs
  • Time Factors
  • Cricetinae
  • Real-Time Polymerase Chain Reaction
• Keywords:
  • RNA stability
  • lysis buffer
  • storage
  • temperature
• Source:
  Journal of Virological Methods, 2025, v. 337
• DOI:
  https://doi.org/10.1016/j.jviromet.2025.115175
• ISSN:
  0166-0934 ; 1879-0984
• Pubmed ID:
  40345598
• Pubmed Central ID:
  PMC12232495
• Document Type:
  Text
• Funding:
  CC999999/ImCDC/Intramural CDC HHSUnited States/ ; US Department of Energy/ ; CC/CDC HHSUnited States/ ; USDA/
• Genre:
  Journal Article
• Pages in Document:
  115175 (10 pdf pages)
• Volume:
  337
• Download URL:
  https://stacks.cdc.gov/view/cdc/261424/cdc_261424_DS1.pdf
• File Type:
  [PDF - 742.93 KB]
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha-512:e35076de9e0ff09bb3a5b04e096271f41f5138ac5c92225956d39e8e0375f7089cfb9291a41b6ec0949b74b80ddc6b8f4fb19da8152951a13a50bfec71e853db